Molecular Biology Reports

, Volume 29, Issue 3, pp 317–323

Screen and identification of proteins interacting with ADAM19 cytoplasmic tail


  • Li Huang
    • Institute of GeneticsFudan University
  • Libing Feng
    • Institute of GeneticsFudan University
  • Limin Yang
    • Institute of GeneticsFudan University
  • Weiguo Zhou
    • Institute of GeneticsFudan University
  • Shouyuan Zhao
    • Institute of GeneticsFudan University
  • Changben Li
    • Institute of GeneticsFudan University

DOI: 10.1023/A:1020409217215

Cite this article as:
Huang, L., Feng, L., Yang, L. et al. Mol Biol Rep (2002) 29: 317. doi:10.1023/A:1020409217215


ADAM family plays important roles in neurogenesis. The cytoplasmic tail of ADAM19 (ADAM19-CT) contains 193 residues. The presence of two putative SH3 ligand-bianding sites suggests potential interactions with cytosolic proteins, which could be possibly linked to the functions of ADAM19. To address these issues, a yeast two-hybrid screen was performed in human fetal brain cDNA library to isolate proteins that interact with the cytoplasmic tail of ADAM19. Four proteins were obtained, ArgBP1, β-cop, ubiquitin and a novel protein. GST-Pulldown assay has confirmed the interaction between AdAM19 and ArgBP1. By constructing series of deletion mutants of ADAM19-CT and ArgBP1 respectively, the interaction regions have been identified. They are the SH3 binding sites in ADAM19-CT and the P4 region in ArgBP1. And the interaction is specific. ArgBP1 does not bind to ADAM22, ADAM29 or ADAM9 (mouse). ArgBP1may be the key protein, which accounts for the physiological function of ADAM19.

ADAMArgBP1yeast two-hybrid

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© Kluwer Academic Publishers 2002