Biphasic Oxygen Kinetics of Cellular Respiration and Linear Oxygen Dependence of Antimycin A Inhibited Oxygen Consumption
- Cite this article as:
- Hütter, E., Renner, K., Jansen-Dürr, P. et al. Mol Biol Rep (2002) 29: 83. doi:10.1023/A:1020322922732
Oxygen kinetics in fibroblasts was biphasic. This was quantitatively explained by a major mitochondrial hyperbolic component in the low-oxygen range and a linear increase of rotenone-and antimycin A-inhibited oxygen consumption in the high-oxygen range. This suggests an increased production of reactive oxygen species and oxidative stress at elevated, air-level oxygen concentrations. The high oxygen affinity of mitochondrial respiration provides the basis for the maintenance of a high aerobic scope at physiological low-oxygen levels, whereas further pronounced depression of oxygen pressure induces energetic stress under hypoxia.