Molecular Biology Reports

, Volume 29, Issue 1, pp 83–87

Biphasic Oxygen Kinetics of Cellular Respiration and Linear Oxygen Dependence of Antimycin A Inhibited Oxygen Consumption

  • Eveline Hütter
  • Kathrin Renner
  • Pidder Jansen-Dürr
  • Erich Gnaiger
Article

DOI: 10.1023/A:1020322922732

Cite this article as:
Hütter, E., Renner, K., Jansen-Dürr, P. et al. Mol Biol Rep (2002) 29: 83. doi:10.1023/A:1020322922732

Abstract

Oxygen kinetics in fibroblasts was biphasic. This was quantitatively explained by a major mitochondrial hyperbolic component in the low-oxygen range and a linear increase of rotenone-and antimycin A-inhibited oxygen consumption in the high-oxygen range. This suggests an increased production of reactive oxygen species and oxidative stress at elevated, air-level oxygen concentrations. The high oxygen affinity of mitochondrial respiration provides the basis for the maintenance of a high aerobic scope at physiological low-oxygen levels, whereas further pronounced depression of oxygen pressure induces energetic stress under hypoxia.

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Eveline Hütter
    • 1
  • Kathrin Renner
    • 2
  • Pidder Jansen-Dürr
    • 1
  • Erich Gnaiger
    • 3
  1. 1.Institute for Biomedical Aging ResearchAustrian Academy of SciencesInnsbruckAustria
  2. 2.Tyrolean Cancer Research InstituteInnsbruckAustria
  3. 3.Department of Transplant Surgery, D. Swarovski Research LaboratoryUniversity Hospital InnsbruckInnsbruck