Euphytica

, Volume 127, Issue 2, pp 209–218

Novel male-specific molecular markers (MADC5, MADC6) in hemp

Authors

  • Ottó Törjék
    • Dept. of Genetics & Plant BreedingSt. István University
  • Nándor Bucherna
    • Dept. of Genetics & Plant BreedingSt. István University
  • Erzsébet Kiss
    • Dept. of Genetics & Plant BreedingSt. István University
  • Hajnalka Homoki
    • Dept. of Genetics & Plant BreedingSt. István University
  • Zsuzsanna Finta-Korpelová
    • Agricultural Research InstituteSt. István University
  • Iván Bócsa
    • Agricultural Research InstituteSt. István University
  • István Nagy
    • Agricultural Biotechnology Center
  • László E. Heszky
    • Dept. of Genetics & Plant BreedingSt. István University
Article

DOI: 10.1023/A:1020204729122

Cite this article as:
Törjék, O., Bucherna, N., Kiss, E. et al. Euphytica (2002) 127: 209. doi:10.1023/A:1020204729122

Abstract

Decamer RAPD primers were tested on dioecious and monoecious hemp cultivars to identify sex-specific molecular markers. Two primers (OPD05 and UBC354) generated specific bands in male plants. These two DNA fragments were isolated, cloned and sequenced. Both markers proved to be unique, since no sequence with significant homology to OPD05961 and UBC354151 markers were found in databases. These markers were named MADC3 (OPD05961) and MADC4 (UBC354151) (Male-Associated DNA from Cannabis sativa). The markers were converted into sequence-characterized amplified region (SCAR) markers. The SCAR markers correlated with the sex of the segregating F2 population and proved the tight linkage to the male phenotype. Results of F2 plant population analysis suggest these markers are to be linked to the Y chromosome.

Cannabis sativaRAPDSCARsex identification
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Copyright information

© Kluwer Academic Publishers 2002