Grape skins as a natural support for yeast immobilization
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Grape skins were used to immobilize Saccharomyces cerevisiae. In repeated batch fermentations of grape by immobilized and free cells, the maximum specific rate of alcohol production on glucose decreased from 7.98 h−1 at 25 °C to 0.7 h−1 at 5 °C. The rate was approximately twice as high as that on fructose. The rates for free cells were very low. The maximum alcohol yield (0.45 g g−1) was obtained at 5 °C when the immobilized biocatalyst was used.
- Bardi EP, Koutinas AA (1994) Immobilization of yeast on delignified cellulosic material for room temperature and lowtemperature wine-making. J. Agric. Food Chem. 42: 221-226.
- Bardi EP, Bakoyianis V, Koutinas AA, Kanellaki M (1996) Room temperature and low temperature wine making using yeast immobilized on gluten pellets. Proc. Biochem. 31: 425-430.
- Brányik T, Vicente AA, Machado C, Teixeira AJ (2001) Spent grains-a new support for brewing yeast immobilization. Biotechnol. Lett. 23: 1073-1078.
- Chen C, Dale CM, Okos RM(1990) Minimal nutrition requirements for immobilized yeast. Biotechnol. Bioeng. 36: 993-1001.
- De Pina GC, Hogg AT (1999) Microbial and chemical changes during the spontaneous ensilage of grape pomace. J. Appl. Microbiol. 86: 777-784.
- Dervakos AG, Webb C (1991) On the merits of viable-cell immobilization. Biotechnol. Adv. 9: 559-612.
- Galazzo LJ, Bailey J (1990) Growing Saccharomyces cerevisiae in calcium-alginate beads induces cell alterations which accelerate glucose conversion to ethanol. Biotechnol. Bioeng. 36: 417-426.
- Gerbsch N, Buchholz R (1995) New processes and actual trends in biotechnology. FEMS Microbiol. Rev. 16: 259-269.
- Hilge-Rotmann B, Rehm JH (1990) Comparison of fermentation properties and specific enzyme activities of free and calciumalginate-entrapped Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 33: 54-58.
- Kourkoutas Y, Komaitis M, Koutinas AA, Kanellaki M(2001) Wine production using yeast immobilized on apple pieces at low and room temperatures. J. Agric. Food Chem. 49: 1417-1425.
- Marín RM (1999) Alcoholic fermentation modeling: current state and perspectives. Am. J. Enol. Vitic. 50: 166-178.
- Pátková J, Šmogrovičová D, Dömény Z, Bafrncová P (2000) Very high gravity wort fermentation by immobilized yeast. Biotechnol. Lett. 22: 1173-1177.
- Shindo S, Takata S, Taguchi H, Yoshimura N (2001) Development of novel carrier using natural zeolite and continuous ethanol fermentation with immobilized Saccharomyces cerevisiae in a bioreactor. Biotechnol. Lett. 23: 2001-2004.
- Starzak M, Krzystek L, Nowicki L, Michalski H (1994) Macroapproach kinetics of ethanol fermentation by Saccharomyces cerevisiae: experimental studies and mathematical modeling. Chem. Eng. J. 54: 221-240.
- Grape skins as a natural support for yeast immobilization
Volume 24, Issue 16 , pp 1331-1335
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- alcoholic fermentation
- grape skins
- mathematical modeling
- Saccharomyces cerevisiae
- yeast immobilization
- Industry Sectors
- Author Affiliations
- 1. Department of Food Science and Technology, University of Athens, Iera Odos 75, Athens, 11855, Greece
- 2. Department of Chemistry, Section of Analytical, Environmental and Applied Chemistry, University of Patras, GR-26500, Patras, Greece
- 3. Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, Athens, 11855, Greece