Biochemical Genetics

, Volume 40, Issue 7, pp 273–282

Cloning and Expression of a Novel Retinoblastoma Binding Protein cDNA, RBBP10

Authors

  • Jin-Zhong Chen
    • State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life SciencesFudan University
  • Quan-Sheng Yang
    • United Gene Holdings, Ltd.
  • Shu Wang
    • State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life SciencesFudan University
  • Xian-Fang Meng
    • United Gene Holdings, Ltd.
  • Kang Ying
    • State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life SciencesFudan University
  • Yi Xie
    • State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life SciencesFudan University
  • Yu-Min Mao
    • State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life SciencesFudan University
Article

DOI: 10.1023/A:1019886918029

Cite this article as:
Chen, J., Yang, Q., Wang, S. et al. Biochem Genet (2002) 40: 273. doi:10.1023/A:1019886918029

Abstract

A 2860-bp cDNA was isolated from a human fetal brain cDNA library by high throughput cDNA sequencing, which encodes a putative protein with 186 amino acids. The putative protein shares 90.7% identity with rat pBOG (3403163) and shares 93.4% identity with human RBBP9 (NPA conserved RB binding domain, L × C × E, located between residue 63 and 68 was recognized. Therefore, it was named RBBP10. Mapviewer analysis locates it on human chromosome 20q11.22. RBBP10 spans about 9.6 kb of the genome and consists of six exons and five introns. RT-PCR revealed that the gene was expressed widely in various human tissues, and the expression level is somewhat higher in tumor tissues than in normal tissues. But subsequent sequencing analysis did not found any mutation of this in tumor tissues. The COS 7 cell transfected with the ORF of RBBP10 showed that the protein was distributed both in the cytoplasm and in the nucleus. Our results suggest that RBBP10 is the orthologue of the rat BOG gene (AF025819) and a paralogue of human RBBP9 (AF039564).

RBBP10humancloneexpression

Copyright information

© Plenum Publishing Corporation 2002