Glycoconjugate Journal

, Volume 18, Issue 7, pp 529–537

O-acetyl sialic acid specific IgM in childhood acute lymphoblastic leukaemia

  • Santanu Pal
  • Mitali Chatterjee
  • Dilip Kumar Bhattacharya
  • Santu Bandhyopadhyay
  • Chhabinath Mandal
  • Chitra Mandal
Article

DOI: 10.1023/A:1019692329568

Cite this article as:
Pal, S., Chatterjee, M., Bhattacharya, D.K. et al. Glycoconj J (2001) 18: 529. doi:10.1023/A:1019692329568

Abstract

Initial studies have revealed an enhanced surface expression of O-acetylated sialoglycoconjugates (O-AcSGs) on lymphoblasts concomitant with high titres of IgG in childhood Acute Lymphoblastic Leukaemia (ALL) (Mandal C, Chatterjee M, Sinha D, Br J Haematol 110, 801–12, 2000). In our efforts to identify disease specific markers for ALL, we have affinity-purified IgM directed against O-AcSGs that reacts with three disease specific O-AcSGs present on membrane proteins derived from peripheral blood mononuclear cells (PBMC) of ALL patients. Antibody specificity towards O-AcSGs was confirmed by selective binding to erythrocytes bearing surface O-AcSGs, decreased binding with de-O-acetylated BSM and following pretreatment with O-acetyl esterase. Competitive inhibition ELISA demonstrated a higher avidity of IgM for O-AcSG than IgG. Flow cytometry demonstrated the diagnostic potential of purified O-AcSA IgM as binding was specific with ALL patients and minimal with other haematological disorders and normal individuals. It therefore may be adopted as a non-invasive approach for detection of childhood ALL. Taken together, the data indicates that carbohydrate epitopes having terminal O-AcSA α2 → 6 GalNAc determinants induce disease specific IgG and IgM, potentially useful molecular markers for childhood ALL.

acute lymphoblastic leukaemia (ALL)O-acetylated sialic acidsminimal residual diseaseIgMantibodies against O-acetylated sialic acids

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Santanu Pal
    • 1
  • Mitali Chatterjee
    • 1
  • Dilip Kumar Bhattacharya
    • 2
  • Santu Bandhyopadhyay
    • 3
  • Chhabinath Mandal
    • 4
  • Chitra Mandal
    • 5
  1. 1.Immunobiology DivisionIndian Institute of Chemical BiologyCalcuttaIndia
  2. 2.Vivekananda Institute of Medical SciencesCalcuttaIndia
  3. 3.Cellular ImmunologyIndian Institute of Chemical BiologyCalcuttaIndia
  4. 4.Protein Design and Engineering DivisionsIndian Institute of Chemical BiologyCalcuttaIndia
  5. 5.Immunobiology DivisionIndian Institute of Chemical BiologyCalcuttaIndia