Glycoconjugate Journal

, Volume 18, Issue 7, pp 529–537

O-acetyl sialic acid specific IgM in childhood acute lymphoblastic leukaemia

Authors

  • Santanu Pal
    • Immunobiology DivisionIndian Institute of Chemical Biology
  • Mitali Chatterjee
    • Immunobiology DivisionIndian Institute of Chemical Biology
  • Dilip Kumar Bhattacharya
    • Vivekananda Institute of Medical Sciences
  • Santu Bandhyopadhyay
    • Cellular ImmunologyIndian Institute of Chemical Biology
  • Chhabinath Mandal
    • Protein Design and Engineering DivisionsIndian Institute of Chemical Biology
  • Chitra Mandal
    • Immunobiology DivisionIndian Institute of Chemical Biology
Article

DOI: 10.1023/A:1019692329568

Cite this article as:
Pal, S., Chatterjee, M., Bhattacharya, D.K. et al. Glycoconj J (2001) 18: 529. doi:10.1023/A:1019692329568

Abstract

Initial studies have revealed an enhanced surface expression of O-acetylated sialoglycoconjugates (O-AcSGs) on lymphoblasts concomitant with high titres of IgG in childhood Acute Lymphoblastic Leukaemia (ALL) (Mandal C, Chatterjee M, Sinha D, Br J Haematol 110, 801–12, 2000). In our efforts to identify disease specific markers for ALL, we have affinity-purified IgM directed against O-AcSGs that reacts with three disease specific O-AcSGs present on membrane proteins derived from peripheral blood mononuclear cells (PBMC) of ALL patients. Antibody specificity towards O-AcSGs was confirmed by selective binding to erythrocytes bearing surface O-AcSGs, decreased binding with de-O-acetylated BSM and following pretreatment with O-acetyl esterase. Competitive inhibition ELISA demonstrated a higher avidity of IgM for O-AcSG than IgG. Flow cytometry demonstrated the diagnostic potential of purified O-AcSA IgM as binding was specific with ALL patients and minimal with other haematological disorders and normal individuals. It therefore may be adopted as a non-invasive approach for detection of childhood ALL. Taken together, the data indicates that carbohydrate epitopes having terminal O-AcSA α2 → 6 GalNAc determinants induce disease specific IgG and IgM, potentially useful molecular markers for childhood ALL.

acute lymphoblastic leukaemia (ALL)O-acetylated sialic acidsminimal residual diseaseIgMantibodies against O-acetylated sialic acids

Copyright information

© Kluwer Academic Publishers 2001