Pharmaceutical Research

, Volume 11, Issue 12, pp 1809–1814

Effect of Electroporation on Transdermal lontophoretic Delivery of Luteinizing Hormone Releasing Hormone (LHRH) in Vitro

Authors

  • D. Bommi Bommannan
    • Cygnus Therapeutic Systems
  • Janet Tamada
    • Cygnus Therapeutic Systems
  • Lewis Leung
    • Cygnus Therapeutic Systems
  • Russell O. Potts
    • Cygnus Therapeutic Systems
Article

DOI: 10.1023/A:1018983804635

Cite this article as:
Bommannan, D.B., Tamada, J., Leung, L. et al. Pharm Res (1994) 11: 1809. doi:10.1023/A:1018983804635

Abstract

Electroporation, the creation of transient, enhanced membrane permeability using short duration (microseconds to millisecond) electrical pulses, can be used to increase transdermal drug delivery. The effect of an (electroporative) electric pulse (1000 V, τ = 5 msec) on the iontophoretic transport of LHRH through human skin was studied in vitro. Fluxes achieved with and without a pulse under different current densities (0- 4 mA/cm2) were compared. The results indicated that the application of a single pulse prior to iontophoresis consistently yielded higher fluxes (5—10 times the corresponding iontophoretic flux). For example, at 0.5 mA/cm2 fluxes were 0.27 ± 0.08 and 1.62 ± 0.05 µg/hr/cm2 without and with the pulse, respectively. At each current density studied, the LHRH flux decreased after iontophoresis, approaching pre-treatment values. The results show that electroporation can significantly and reversibly increase the flux of LHRH through human skin. These results also indicate the therapeutic utility of using electroporation for enhanced transdermal transport.

transdermal drug deliveryLHRHiontophoresiselectroporationelectropermeabilizationelectrotransport

Copyright information

© Plenum Publishing Corporation 1994