Percutaneous Absorption of Sunscreens Through Micro-Yucatan Pig Skin In Vitro
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Purpose. The objectives of this study were to develop an in vitro model for studying sunscreen permeation in skin, and evaluate the influence of formulation differences.
Methods. The sunscreens studied were two of the most widely used agents, octyl methoxycinnamate (OMC) and benzophenone-3. Preparations containing radiolabeled actives were applied to micro-Yucatan pig skin dermatomed to a thickness of 250−300 μm as a finite dose in a flow-through diffusion system. At the end of each experiment the amounts removed by washing, retained inside stratum corneum (SC) and penetrated into receptor and viable skin were determined.
Results. The two sunscreens reached a peak level in SC within an hour. Benzophenone-3 penetrated skin to a greater extent than OMC. The opposite was true when comparisons of SC retention were made. The ratio of retained to penetrated amount of sunscreens from a hydroalcoholic formulation at the end of 10 hours was higher when the sunscreens were present together than alone.
Conclusions. Despite the highly lipophilic nature of sunscreens, particularly OMC, SC is the rate limiting skin layer for penetration. Penetration and SC retention were formulation dependent. The ratio of SC content to the amount penetrated is a useful tool for evaluating sunscreen permeation.
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- Percutaneous Absorption of Sunscreens Through Micro-Yucatan Pig Skin In Vitro
Volume 16, Issue 10 , pp 1602-1607
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- in vitro percutaneous absorption
- octyl methoxycinnamate
- retention inside stratum corneum
- Industry Sectors
- Author Affiliations
- 1. Organon Inc., 375 Mt. Pleasant Ave., West Orange, New Jersey, 07052
- 2. Department of Pharmaceutics, Rutgers University, College of Pharmacy, 160 Frelinghuysen Road, Piscataway, New Jersey, 08854
- 3. Skin Care, ISP, Wayne, New Jersey, 07470