Pharmaceutical Research

, Volume 10, Issue 10, pp 1427–1433

Inhibition of the Friend Retrovirus by Antisense Oligonucleotides Encapsulated in Liposomes: Mechanism of Action

  • C. Ropert
  • C. Malvy
  • P. Couvreur
Article

DOI: 10.1023/A:1018910922633

Cite this article as:
Ropert, C., Malvy, C. & Couvreur, P. Pharm Res (1993) 10: 1427. doi:10.1023/A:1018910922633

Abstract

Proliferation of the Friend retrovirus was specifically inhibited by the env mRNA complementary oligonucleotide encapsulated in pH-sensitive liposomes. This observation was made using the focus immunoassay (FIA) and the reverse transcriptase test. The key finding of the present study was the dramatic impact on liposome penetration. For chronic or de novo infection, the point at which the penetration of liposomes began corresponded to the time needed for the virus to leave the cell. In the absence of the virus, liposomes remained adsorbed onto the cell surface without any internalization. Regardless of the mechanism involved, the fact that a retroviral infection stimulates the cellular uptake of oligonucleotide liposomes widens the spectrum of strategies for specific antiviral action.

Friend retroviruspH-sensitive liposomesoligonucleotidesfibroblastsfocus immunoassay

Copyright information

© Plenum Publishing Corporation 1993

Authors and Affiliations

  • C. Ropert
    • 1
  • C. Malvy
    • 1
  • P. Couvreur
    • 2
  1. 1.URA 147 CNRS, U140 INSERM, Institut Gustave RoussyVillejuif CedexFrance
  2. 2.URA 1218, Université Paris 11, Centre d'Etudes Pharmaceutiques92296Chatenay-Malabry CedexFrance