Inhibition of mitochondrial calcium uptake slows down relaxation in mitochondria-rich skeletal muscles
- J. M. GILLIS
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Isolated fibres from various muscles were skinned mechanically in oil. From a Ca2+-loaded micropipette, local applications of Ca2+ were made. These produced a limited contraction which relaxed spontaneously. The time-course of sarcomere shortening and re-lengthening was recorded by microcinephotography. Application of Ruthenium Red, a potent and specific inhibitor of Ca2+ uptake by mitochondria, did not affect the contraction- relaxation cycles of typical glycolytic white fibres (frog sartorius, pigeon breast). By contrast, Ruthenium Red greatly slowed down the relaxation rate in mitochondria-rich fibres (rat soleus and rabbit masseter). In these fibres, Ca2+ uptake by mitochondria seems to play an active role in promoting relaxation
- ASHLEY, C. C., CALDWELL, P. C., LOWE, A. G., RICHARDS, C. D. & SCHIRMER, M. (1965) The amount of injected EGTA needed to suppress the contractile response of single Maia muscle fibres and its relation to the amount of calcium released during contraction. J. Physiol. 179, 32–33.
- ASHLEY, C. C., MOISESCU, D. G. & ROSE, R. M. (1974) Kinetics of calcium during contraction: myofibrillar and SR fluxes during a single response of a skeletal muscle fibre. In Calcium binding proteins (edited by DRABIKOWSKI, W., STRZELECKA-GOLASZEWSKA, M. & CARAFOLI, E.) pp. 609–42. Amsterdam: Elsevier.
- BAKKER, A. J., HEAD, S. I. & STEPHENSON, D. G. (1997) Time course of calcium transients derived from Fura-2 fluorescence measurements in single fast twitch fibres of adult mice and rat myotubes developing in primary culture. Cell Calcium 21, 359–64
- BAYLOR, S. M., CHANDLER, W. K. & MARSHALL, M. W. (1983) Sarcoplasmic reticulum calcium release in frog skeletal muscle fibres estimated from arsenazo III calcium transients J. Physiol. 344, 625–66.
- BLINKS, J. R., RÜDEL, R. & TAYLOR, S. R. (1978) Calcium transients in isolated amphibian muscle fibres: detection with aequorin. J. Physiol. 277, 291–323.
- CARDOSO, C. M. & DE MEIS, L. (1993) Modulation by fatty acids of Ca2+ fluxes in sarcoplasmic reticulum vesicles. Biochem. J. 296, 49–52.
- CARAFOLI, E. (1987) Intracellular calcium homeostasis Ann. Rev. Biochem. 56, 395–433.
- CARROLL, S. L., KLEIN, M. G. & SCHNEIDER, M. F. (1995) Calcium transient in intact rat skeletal muscle fibers in agarose gel. Am. J. Physiol. 269, C28–34.
- CHACON, E., OHATA, H., HARPER, I. S., TROLLINGER, D. R., HERMAN, B. & LEMASTERS, J. J. (1996) Mitochondria free calcium transients during excitation-contraction coupling in rabbit cardiac myocytes. FEBS Lett. 382, 31–6.
- CLOSE, R. I. (1972) Dynamic properties in mammalian skeletal muscles. Physiol. Rev. 52, 129–97.
- CROMPTON, N., SIGEL, E., SALZMANN, M. & CARAFOLI, E. (1976) A kinetic study of the energy-linked influx of Ca2+ into heart mitochondria Eur. J. Biochem. 69, 429–34.
- DELBONO, O. & STEPHANI, E. (1993) Calcium transients in single mammalian skeletal muscle fibres. J. Physiol. 463, 689–707.
- FRYER, M. W. & NEERING, I.R. (1989) Actions of caffeine on fast and slow twitch muscles of the rat. J. Physiol. 416, 435–54.
- GEORGE, J. C. & BERGER, A. J. (1966) Avian Myology. New York: Academic Press.
- GILLIS, J. M. (1985) Relaxation of vertebrate skeletal muscle. A synthesis of the biochemical and physiological approaches Biophys. Biochim. Acta 811, 97–145.
- GILLIS, J. M., MAES, M. & VERELLEN-DUMOULIN, C. (1973) Controlled application of calcium to sarcolemma-free muscle fibres J. Physiol. 232, 1–3P.
- GUNTER, T. E. & PFEIFFER, D. R. (1990) Mechanisms by which mitochondria transport calcium Am. J. Physiol. 258, C755–86.
- HAJNÓCZKY, G., ROBB-GASPERS, L. D., SEITZ, M. B. & THOMAS, A. P. (1995) Decoding of cytosolic calcium oscillations in the mitochondria. Cell 82, 415–24.
- LÄNNERGREN, J. (1971) The effect of low level activation on the mechanical properties of isolated frog muscle fibers. J. Gen. Physiol. 58, 145–62.
- MOENS, P., PARTRIDGE, T. A., MORGAN, J. E., BECKERSBLEUKS, G. & MARÉCHAL, G. (1992) Regeneration after free muscle grafting in normal and dystrophic (mdx) mice. J. Neurol. Sci. 111, 209–13.
- MOORE, C. L. (1971) Specific inhibition of mitochondrial Ca2+ transport by ruthenium red. Biochem. Biophys. Res. Com., 42, 298–305.
- PODOLSKY, R. J. (1964) The maximum sarcomere length for contraction of isolated myofibrils J. Physiol. 170, 110–23.
- RIZZUTO, R., SIMPSON, A. W. M., BRINI, M. & POZZAN, T. (1992) Rapid changes of mitochondrial Ca2+ revealed by specifically targeted recombinant aequorin. Nature, 358325–7.
- RUTTER, G. A., THELER, J. M., MURGIA, M., WOLLHEIM, C. B., POZZAN, T. & RIZZUTO, R. (1993) Stimulated Ca2+ influx raises mitochondrial free Ca2+ to supramicromolar levels in pancreatic β-cell line. J. Biol. Chem. 268, 22385–90.
- SCIOTE, J. J. & KENTISH, J. C. (1996) Unloaded shortening velocities of rabbit masseter muscle fibres expressing skeletal or α-cardiac myosin heavy chains. J. Physiol. 492, 659–67.
- WESTERBLAD, H. & ALLEN, D. G. (1991) Changes of myoplasmic calcium concentration during fatigue in single mouse muscle fibers. J. Gen. Physiol. 98, 615–35.
- WESTERBLAD, H. & ALLEN, D. G. (1995) Relaxation, [Ca2+]i and [Mg2+]i during prolonged tetanic stimulation of intact, single fibres from mouse skeletal muscle. J. Physiol. 480, 31–43.
- WOLEDGE, R. C., CURTIN, N. A. & HOMSHER, E. (1985) In Energetics Aspects of Muscle Contraction. Monographs of the Physiol. Soc, 41, pp. 97–8. London: Academic Press.
- Inhibition of mitochondrial calcium uptake slows down relaxation in mitochondria-rich skeletal muscles
Journal of Muscle Research & Cell Motility
Volume 18, Issue 4 , pp 473-483
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- Kluwer Academic Publishers
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- J. M. GILLIS (1)
- Author Affiliations
- 1. Department of Physiology, Catholic University of Louvain, Faculty of Medicine, UCL 5540, 55 avenue Hippocrate, 1200, Bruxelles, Belgium