Cellular mechanisms of pH tolerance in Rhizobium loti
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Seven strains of Rhizobium loti were tested for acid tolerance in yeast-extract mannitol (YEM) broth at pH values ranging from 4.0 to 8.0. The strains that grew at pH 4.0 showed the slowest generation time when grown at pH above 7.0 and also produced the most acid. The acid tolerance was related to the composition and structure of the membrane. pH influenced protein expression in acid-tolerant strains growing at pH 4.0 or 7.0. Acid tolerant strains showed one membrane protein of 49.5 kDa and three soluble proteins of 66.0, 58.0 and 44.0kDa; their expression increased when the cells grew at pH 4.0. It is suggested that acid tolerance in Rhizobium loti involves constitutive mechanisms, such as permeability of the outer membrane together with adaptive responses, including the state of bacterial growth and concomitant changes in protein expression.
- Aarons, S.R. & Graham, P.H. 1991 Response of Rhizobium leguminosarum bv phaseoli to acidity. Plant & Soil 134, 145–151.
- Bordeleau, L.M. & Prévost, D. 1994 Nodulation and nitrogen fixation in extreme environments. Plant & Soil 161, 115–124.
- Brockwell, J., Pilka, A. & Holliday, R.A. 1991 Soil pH is a major determinant of numbers of naturally occurring Rhizobium meliloti in non-cultivated soils in central New South Wales. Australian Journal of Experimental Agriculture 31, 211–219.
- Chen, H., Richardson, A.E. & Rolfe, B.G. 1993 Studies of the physiological and genetic basis of acid tolerance in Rhizobium leguminosarum biovar trifolii. Applied and Environmental Microbiology 59, 1798–1804.
- Cunningham, S.D. & Munns, D.N. 1984 The correlation between extracellular polysaccharide production and acid tolerance in Rhizobium. Soil Science Society of America Journal 48, 1273–1276.
- Glenn, A.R. & Dilworth, M.J. 1994 The life of root nodule bacteria in the acidic underground. FEMS Microbiology Letters 123, 1–10.
- Graham, P.H., Draeger, K.J., Ferrey M.L., Conroy, M.J., Hammer, B.E., Martínez, E., Aarons, S.R. & Quinto, C. 1994 Acid pH tolerance in strains of Rhizobium and Bradyrhizobium, and initial studies on the basis for acid tolerance of Rhizobium tropici UMR 1899. Canadian Journal of Microbiology 40, 198–207.
- Kolter, R., Siegele, D.A. & Tormo A. 1993 The stationary phase of the bacterial life cycle. Annual Review of Microbiology 47, 855– 874.
- Laemmli, U.K. 1970 Cleavage of Structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
- Leyer, G.J. & Johnson, E.A. 1993 Acid adaptation induces cross-protection against environmental stresses in Salmonella typhimurium. Applied and Environmental Microbiology 59, 1842–1847.
- O'Hara, G.W. & Glenn, A.R. 1994 The adaptive acid tolerance response in root nodule bacteria and Escherichia coli. Archives of Microbiology 161, 286–292.
- Miñón. P.D., Sevilla, G.H., Montes, L. P. & Fernandez, O. 1990 Lotus tenuis Leguminosa forrajera para la pampa deprimida Boletin técnico n° 98. Unidad Integrada INTA Balcarce.
- Rice, W.A., Penney, D.C. & Nyborg, M. 1977 Effects of soil acidity on rhizobia numbers, nodulation and nitrogen fixation by alfalfa and red clover. Canadian Journal of Soil Science 57, 197–203.
- Tiwari, R.P., Reeve, W. G. & Glenn, A.R. 1992 Mutations conferring acid sensitivity in the acid-tolerant strains Rhizobium meliloti WSM419 and Rhizobium leguminosarum biovar viciae WSM710. FEMS Microbiology Letters 100, 107–112.
- Vincent, J.M. 1970 A Manual for the Practical Study of Root-Nodule Bacteria. Oxford: Blackwell Scientific Publications.
- Wood, M., Cooper, J.E. & Bjourson, A.J. 1988 Response of Lotus rhizobia to acidity and aluminium in liquid culture and in soil. Plant and Soil 107, 227–231.
- Cellular mechanisms of pH tolerance in Rhizobium loti
World Journal of Microbiology and Biotechnology
Volume 13, Issue 2 , pp 153-157
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