Glycoconjugate Journal

, Volume 14, Issue 5, pp 549–560

Specificities of anti-sialyl-Tn and anti-Tn monoclonal antibodies generated using novel clustered synthetic glycopeptide epitopes

  • Mark A Reddish
  • Linda Jackson
  • R Rao Koganty
  • Dongxu Qiu
  • Wu Hong
  • B Michael Longenecker
Article

DOI: 10.1023/A:1018576224062

Cite this article as:
Reddish, M.A., Jackson, L., Rao Koganty, R. et al. Glycoconj J (1997) 14: 549. doi:10.1023/A:1018576224062

Abstract

The fine specificities of MAbs generated using novel synthetic clustered STn and Tn glycopeptides as immunogens were compared with the anti-TAG-72 antibodies B72.3 and CC49. Hapten inhibition experiments demonstrated the specificity of several of the MAbs for STn and Tn expressed on ovine submaxillary mucin and tumor derived MUC-1 mucin. Amongst the STn specific MAbs only the B195.3 MAb shows absolute dependence on the presence of sialic acid and specificity to the simple disaccharide NANAA α2-6-GalNAc. Identification of tumor associated carbohydrate epitopes in cluster and monomer configurations are possible using MAbs detecting the defined structure specificities described herein.

sialyl-TnTn MUC-1cancer mucin

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • Mark A Reddish
    • 1
  • Linda Jackson
    • 1
  • R Rao Koganty
    • 1
  • Dongxu Qiu
    • 1
  • Wu Hong
    • 2
  • B Michael Longenecker
    • 3
  1. 1.Biomira Inc.EdmontonCanada
  2. 2.Faculty of Pharmacy and Pharmaceutical SciencesUniversity of AlbertaEdmontonCanada
  3. 3.Department of ImmunologyUniversity of AlbertaEdmontonCanada