, Volume 14, Issue 1, pp 75-80

Enzymatic syntheses of GlcNAcβ1-2Man and Galβ1-4GlcNAcβ1-2Man as components of complex type sugar chains

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GlcNAcβ1-2Man and GlcNAcβ1-6Man were synthesized using the reverse hydrolysis activity of β-N-acetylglucosaminidase from both jack beans and Bacillus circulans. In turn, Galβ1-4GlcNAcβ1-2Man and Galβ1-4GlcNAcβ1-6Man were synthesized regioselectively using the transglycosylation activity of β-galactosidase from Diplococcus pneumoniae and B. circulans, respectively. These di- and trisaccharides are important components of complex type sugar chains and will be used as intermediates in our synthetic studies. Abbreviations: pNp-β-GlcNAc, p-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside; pNp-β-Gal, p-nitrophenyl β-D-galacto-pyranoside