Fermentation of corn fibre sugars by an engineered xylose utilizing Saccharomyces yeast strain
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The ability of a recombinant Saccharomyces yeast strain to ferment the sugars glucose, xylose, arabinose and galactose which are the predominant monosaccharides found in corn fibre hydrolysates has been examined. Saccharomyces strain 1400 (pLNH32) was genetically engineered to ferment xylose by expressing genes encoding a xylose reductase, a xylitol dehydrogenase and a xylulose kinase. The recombinant efficiently fermented xylose alone or in the presence of glucose. Xylose-grown cultures had very little difference in xylitol accumulation, with only 4 to 5g/l accumulating, in aerobic, micro-aerated and anaerobic conditions. Highest production of ethanol with all sugars was achieved under anaerobic conditions. From a mixture of glucose (80g/l) and xylose (40g/l), this strain produced 52g/l ethanol, equivalent to 85% of theoretical yield, in less than 24h. Using a mixture of glucose (31g/l), xylose (15.2g/l), arabinose (10.5g/l) and galactose (2g/l), all of the sugars except arabinose were consumed in 24h with an accumulation of 22g ethanol/l, a 90% yield (excluding the arabinose in the calculation since it is not fermented). Approximately 98% theoretical yield, or 21g ethanol/l, was achieved using an enzymatic hydrolysate of ammonia fibre exploded corn fibre containing an estimated 47.0g mixed sugars/l. In all mixed sugar fermentations, less than 25% arabinose was consumed and converted into arabitol.
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- Fermentation of corn fibre sugars by an engineered xylose utilizing Saccharomyces yeast strain
World Journal of Microbiology and Biotechnology
Volume 13, Issue 3 , pp 341-346
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
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- corn fibre
- Industry Sectors
- Author Affiliations
- 1. Department of Chemical Engineering, Texas A&M University, College Station, TX, 77843, USA
- 2. National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, Fermentation Biochemistry Research Unit, 1815 N. University Street, Peoria, IL, 61604, USA
- 3. Department of Chemical Engineering, Michigan State University, East Lansing, MI, 48824, USA
- 4. LORRE, Purdue University, 1295 Potter Center, West Lafayette, IN, 47907, USA