Biotechnology Letters

, Volume 19, Issue 10, pp 1027–1031

Cloning and nucleotide sequence of a thermostable cyclodextrin glycosyltransferase gene from Thermoanaerobactersp. ATCC 53627 and its expression in Escherichia coli

  • S.T. Jørgensen
  • M. Tangney
  • R.L. Starnes
  • K. Amemiya
  • P.L. Jørgensen
Article

DOI: 10.1023/A:1018459703742

Cite this article as:
Jørgensen, S., Tangney, M., Starnes, R. et al. Biotechnology Letters (1997) 19: 1027. doi:10.1023/A:1018459703742

Abstract

A gene, cgtA, encoding an extremely thermostable cyclodextrin glycosyltransferase (CGTase) was cloned from a thermophilic anaerobe, Thermoanaerobacter sp. ATCC 53627, and expressed in Escherichia coli. DNA and protein sequencing revealed that the mature enzyme of 683 amino acid residues (MW 75 kDa) was preceded by a signal peptide of 27 amino acid residues. The sequence of the Thermoanaerobacter CGTase was similar to sequences of Bacillus CGTases, with more than 58% identity, and very similar (89% identity) to a CGTase enzyme from Thermoanaerobacterium thermosulfurogenes.

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • S.T. Jørgensen
    • 1
  • M. Tangney
    • 2
  • R.L. Starnes
    • 3
    • 4
  • K. Amemiya
  • P.L. Jørgensen
    • 1
  1. 1.Department of Bacterial Gene TechnologyNovo Nordisk A/SBagsvaerdDenmark
  2. 2.Biological Sciences DepartmentHeriot-Watt University, Riccarton EdinburghScotlandUK
  3. 3.NN Entotech, Inc.DavisUSA
  4. 4.National Institutes of HealthBethesdaUSA

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