Clinical & Experimental Metastasis

, Volume 15, Issue 2, pp 94–101

Enhancement of lung-colonizing potential of murine tumor cell lines co-cultivated with activated macrophages

Authors

  • Oliviero Cecconi
    • Institute of General Pathology of the University of Florence
  • Lido Calorini
    • Institute of General Pathology of the University of Florence
  • Antonella Mannini
    • Institute of General Pathology of the University of Florence
  • Gabriele Mugnai
  • Salvatore Ruggieri
    • Institute of General Pathology of the University of Florence
Article

DOI: 10.1023/A:1018440508189

Cite this article as:
Cecconi, O., Calorini, L., Mannini, A. et al. Clin Exp Metastasis (1997) 15: 94. doi:10.1023/A:1018440508189

Abstract

In order to explore the influence of activated macrophages on tumor cells, we cultured a series of weakly metastatic clones isolated from the murine T3 fibrosarcoma line (T3 clones) and the B16-F10 melanoma cells on feeder layers of C. parvum- or thioglycollate-elicited macrophages, or ÔresidentÕ (unstimulated) macrophages. Co-cultivation with C. parvum-elicited macrophages, but not with resident macrophages, induced an increase of the lung-colonizing potential of T3 clones and B16-F10 cells. An enhancement of lung-colonizing potential was also found in tumor cells grown in media conditioned by C. parvum-elicited macrophages. Thioglycollate-elicited macrophages also generated a pro-clonogenic activity which was however effective only on T3 clones but not on B16-F10 cells. The increase in the lung-colonizing potential of tumor cells stimulated by activated macrophages was retained to some degree after subcultivation in tissue culture medium or transplantation into syngeneic animals. In conclusion, our data support the notion that macrophages at different states of activation may enhance lung colonization of tumor cells by inducing a partially stable change of phenotype.

C. parvum-elicited macrophagesthioglycollate-elicited macrophagesmurine fibrosarcoma linesB16-F10 melanoma cellslung colonies

Copyright information

© Chapman and Hall 1997