Chromosome Research

, Volume 5, Issue 1, pp 23–30

High-resolution fluorescence in situ hybridization of human Y-linked genes on released chromatin

Authors

  • Birgitta Gla¨ser
    • Institute of Human Genetics and AnthropologyUniversity of Freiburg
  • Thomas Hierl
    • Institute of Human Genetics and AnthropologyUniversity of Freiburg
  • Kay Taylor
    • The Galton LaboratoryUniversity College London
  • Katrin Schiebel
    • Institute of Human Genetics and AnthropologyUniversity of Heidelberg
  • Sylvia Zeitler
    • Institute of Human Genetics and AnthropologyUniversity of Freiburg
  • Katia Papadopoullos
    • The Galton LaboratoryUniversity College London
  • Gudrun Rappold
    • Institute of Human Genetics and AnthropologyUniversity of Heidelberg
  • Werner Schempp
    • Institute of Human Genetics and AnthropologyUniversity of Freiburg
Article

DOI: 10.1023/A:1018437301461

Cite this article as:
Gla¨ser, B., Hierl, T., Taylor, K. et al. Chromosome Res (1997) 5: 23. doi:10.1023/A:1018437301461

Abstract

Genes within the differential region of the human Y chromosome do not recombine, and therefore the determination of their location depends on physical mapping. Yeast artificial chromosome (YAC) contigs spanning the euchromatic region of the human Y have become a powerful tool for the generation of an overlapping clone map. With this approach,however, complete physical mapping is difficult in Y euchromatic regions that are rich in repetitive sequences. We have, therefore, made use of the fluorescence in situ hybridization technique as an alternative strategy for physically mapping the PRKY and AMELY genes as well as the TSPY, RBM and DAZ gene families to human Y chromosomes in prometaphase and to extended Y chromatin in interphase. From our results, the following order of gene sequences in interval 3 of the short arm of the human Y chromosome is suggested: TSPY major with few RBM sequences interspersed-PRKY-AMELY-TSPY minor with few RBM sequences interspersed-cen. On the long arm, RBM sequences appear to be distributed over wide regions of intervals 5 and 6 with few TSPY sequences interspersed. Distal to an RBM signal cluster, a large cluster of DAZ signals is located with only a few DAZ and RBM signals overlapping in between the two clusters.

high-resolution fluorescence in situ hybridizationhuman Y-linked genes

Copyright information

© Chapman and Hall 1997