Abstract
The effect of benzyladenine (BA) and ascorbic acid (AA) on relative water content, proline accumulation, net photosynthetic rate (PN), chlorophyll (Chl) content and nitrate reductase (NR) activity, under sufficient water supply and moisture stress was studied in senna (Cassia angustifolia Vahl.) at seedling, vegetative, flowering and pod formation stages. AA treatment resulted in a higher accumulation of proline at all the stages of growth. Both BA and AA enhanced PN, Chl content and NR activity, and ameliorate the negative effect of water stress.
References
Aspinall, D., Paleg, L.G.: Proline accumulation: physiological aspect.-In: Paleg, L.G., Aspinall, D. (ed.): The Physiology and Biochemistry of Drought Resistance in Plants. Pp. 206–241, Academic Press, Sydney 1981.
Barrs, H.D., Weatherley, P.E.: A re-examination of the relative turgidity technique for stimulating water deficits in leaves.-Aust. J. biol. Sci. 15: 413–423, 1962.
Bates, L.S., Waldren, R.P., Tearc, L.D.: Rapid determination of free proline for water stress study.-Plant Soil 39: 205–208, 1973.
Chinoy, J.J., Patel, H.D., Jani, B.M., John, D., Vyas, A.V., Raj, K.P.S.: Physiological Studies of Drought Resistance in some Indian and American Varieties of Barley. (A report on P.L. 480 Research Project.)-ICAR, New Delhi 1969.
Dong, Y.H, Shi, J.P, Li, G.M.: Effect of ABA and 6-BA on PEP carboxylase activity in maize seedlings under soil drought.-Plant Physiol. Commun. 31: 421–423, 1995.
Fedina, I.S., Popova, A.V.: Photosynthesis, photorespiration and proline accumulation in water-stressed pea leaves.-Photosynthetica 32: 213–220, 1996.
Gzik, A., Schonfeld, G., Baumann, I., Gunther, G., Lilov, D.: Plant growth regulators.-In: Vassilev, G., Cristav, C., Andonova, T. (ed.): Proceeding of the IV International Symposium of Plant Growth Regulators. Pp. 547–551. M. Popov Institute of Plant Physiology, Sofia 1987.
Hageman, R.H, Hucklishy, D.P.: Nitrate reductase for higher plants.-Methods Enzymol. 23: 491–498, 1971.
Hiscox, J.D., Israelstam, G.F.: A method for extraction of chlorophyll from leaf tissue without maceration.-Can. J. Bot. 57: 132–134, 1979.
Kishor, P.B.K., Hong, Z.L., Miao, G.H., Hu, C.A.A., Verma, D.P.S.: Overexpression of Δ-1-pyroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants.-Plant Physiol. 108: 1387–1394, 1995.
Krishnayya, G.R., Murty, K.S.: Amelioration of drought injury in rice by chemical spray.-Curr. Sci. 48: 264–265, 1979.
Prabha, C., Bharti, S.: Effect of ascorbic acid on proline accumulation in cowpea leaves under moisture stress conditions.-Indian J. Plant Physiol. 23: 317–318, 1980.
Singh, T.N., Aspinall, D., Paleg, L.G.: Proline accumulation and varietal adaptability to drought in barley: a potential metabolic measure of drought resistance.-Nature 236: 188–190, 1972.
Stewart, C.E., Hanson, A.D.: Proline accumulation as metabolic response to water stress.-In: Turner, N.C., Kramer, P.J. (ed.): Adaptation of Plants to Water and High Temperature. Stress. Pp. 173–189. John Wiley & Sons, New York 1980.
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Singh, D., Srivastava, G. & Abdin, M. Amelioration of Negative Effect of Water Stress in Cassia Angustifolia by Benzyladenine and/or Ascorbic Acid. Biologia Plantarum 44, 141–143 (2001). https://doi.org/10.1023/A:1017955328875
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DOI: https://doi.org/10.1023/A:1017955328875