Chromosome Research

, Volume 9, Issue 5, pp 387–393

Heterogeneity of rDNA distribution and genome size in Silene spp.

  • Jiří Široký
  • Martin A. Lysák
  • Jaroslav Doležel
  • Eduard Kejnovský
  • Boris Vyskot
Article

DOI: 10.1023/A:1016783501674

Cite this article as:
Široký, J., Lysák, M.A., Doležel, J. et al. Chromosome Res (2001) 9: 387. doi:10.1023/A:1016783501674

Abstract

Genus Silene L. (Caryophyllaceae) contains about 700 species divided into 44 sections. According to recent taxonomic classification this genus also includes taxa previously classified in genera Lychnis and Melandrium. In this work, four Silene species belonging to different sections were studied: S. latifolia (syn. Melandrium album, Section Elisanthe), S. vulgaris (Inflatae), S. pendula (Erectorefractae), and S. chalcedonica (syn. Lychnis chalcedonica, Lychnidiformes). Flow cytometric analysis revealed a genome size of 2.25 and 2.35 pg/2C for S. vulgaris and S. pendula and of 5.73 and 6.59 pg/2C for S. latifolia and S. chalcedonica. All four species have the same chromosome number including the pair of sex chromosomes of the dioecious S. latifolia (2n=2x=24). Double target fluorescence in-situ hybridization revealed the chromosomal locations of 25S rDNA and 5S rDNA. A marked variation in number and localization of rDNA loci but no correlation between the numbers of rDNA clusters and genome size was found. FISH and genome size data indicate that nuclear genomes of Silene species are highly diversified as a result of numerous DNA amplifications and translocations.

chromosomecytotaxonomyflow cytometryfluorescencein-situ hybridizationribosomal DNASilene

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Jiří Široký
    • 1
  • Martin A. Lysák
    • 2
  • Jaroslav Doležel
    • 2
  • Eduard Kejnovský
    • 1
  • Boris Vyskot
    • 1
  1. 1.Institute of BiophysicsAcademy of Sciences of the Czech RepublicBrnoCzech Republic
  2. 2.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicOlomoucCzech Republic