Russian Journal of Plant Physiology

, Volume 48, Issue 5, pp 628–632

Oligosaccharide Inhibits Ethylene Synthesis and Stimulates Somatic Embryogenesis in a Cotton Cell Culture

  • V. Yu. Rakitin
  • Yu. I. Dolgikh
  • E. Yu. Shaikina
  • Vl. V. Kuznetsov
Article

DOI: 10.1023/A:1016760019737

Cite this article as:
Rakitin, V.Y., Dolgikh, Y.I., Shaikina, E.Y. et al. Russian Journal of Plant Physiology (2001) 48: 628. doi:10.1023/A:1016760019737

Abstract

The effect of a three-component oligosaccharide fragment of xyloglucan FucGalXyl (XG3) on callus-tissue growth and somatic embryogenesis was investigated in a cotton (Gossypium hirsutumL.) cell suspension culture. The oligosaccharide introduced into an induction medium at 10–8and 10–7M concentrations did not affect the frequency of callus formation from hypocotyl segments; however, it enhanced the monthly increment of callus-tissue weight 1.5- and 3-fold, respectively. Induction and culturing of the callus on an XG3-containing medium adversely affected its morphogenetic potential. Addition of XG3 to the culture medium during the cell suspension preparation stimulated cell division resulting, after 40 days, in a 3.4-fold (at 10–8M XG3) and a 1.7-fold (at 10–7M XG3) increase in the cell number as compared to the control. Exclusion of 2,4-D, kinetin, and oligosaccharide from the culture medium caused, after two weeks, a 3.8-fold increase in the number of embryos in the 10–7M XG3-treated suspension culture as compared to the control. The stimulation of somatic embryogenesis by the oligosaccharide was accompanied by a 12-fold decrease in ethylene emission. The morphogenetic effect of oligosaccharide is suggested to result from its anti-auxin action, which, in particular, inhibited the auxin-dependent ethylene synthesis.

Gossypium hirsutumoligosaccharinsethylenesomatic embryogenesiscallussuspension

Copyright information

© MAIK “Nauka/Interperiodica” 2001

Authors and Affiliations

  • V. Yu. Rakitin
    • 1
  • Yu. I. Dolgikh
    • 1
  • E. Yu. Shaikina
    • 1
  • Vl. V. Kuznetsov
    • 1
  1. 1.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia