Chromosome Research

, Volume 9, Issue 5, pp 395–402

Highly comprehensive karyotype analysis by a combination of spectral karyotyping (SKY), microdissection, and reverse painting (SKY-MD)

  • Jörg Weimer
  • Michael R. Koehler
  • Ute Wiedemann
  • Peter Attermeyer
  • Anja Jacobsen
  • Doris Karow
  • Marion Kiechle
  • Walter Jonat
  • Norbert Arnold
Article

DOI: 10.1023/A:1016735618513

Cite this article as:
Weimer, J., Koehler, M.R., Wiedemann, U. et al. Chromosome Res (2001) 9: 395. doi:10.1023/A:1016735618513

Abstract

A technique disclosing most information about chromosome modifications is the technique of choice for the analysis of chromosome alterations. The newly developed method for microdissection of fluorescence-labeled chromosomes (FISH-MD) can improve upon this expectation in combination with 24-color spectral karyotyping (SKY). The highly efficient way to detect chromosome modifications by SKY and the detailed specification of aberrant chromosomes by FISH-MD prompted us to use both techniques'in a combined approach called SKY-MD. First, an overview of chromosomal aberrations is obtained by spectral karyotyping and subsequently the derivative chromosomes recognized are characterized in a highly specific manner by microdissection and reverse painting. A small quantity of isolated material dissected directly from a 24-color metaphase is sufficient to obtain very detailed information about the chromosome regions and the breakpoints involved in the derivative chromosomes. Therefore, the combination of spectral karyotyping and microdissection in one procedure, and reverse painting can characterize chromosomal aberrations with a degree of specificity hitherto unknown from individual karyotyping experiments. In this article we compare the efficiency of both the SKY technique and that of classical microdissection with the efficiency obtained by SKY-MD.

chromosomechromosome paintingfluorescencein-situ hybridizationmicrodissectionspectral karyotyping

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Jörg Weimer
    • 1
  • Michael R. Koehler
    • 2
  • Ute Wiedemann
    • 3
  • Peter Attermeyer
    • 4
  • Anja Jacobsen
    • 1
  • Doris Karow
    • 1
  • Marion Kiechle
    • 1
    • 5
  • Walter Jonat
    • 1
  • Norbert Arnold
    • 1
  1. 1.Oncology Laboratory, Gynecology and Obstetrics ClinicChristian-Albrechts University KielKiel (Germany
  2. 2.Applied Spectral Imaging GmbHMannheim (Germany
  3. 3.Department of Medical GeneticsUniversity of ZürichZürich (Switzerland
  4. 4.Carl Zeiss Jena GmbHGöttingen (Germany
  5. 5.Department of Gynecology and ObstetricsTechnique University MunichMunich (Germany