Objective Assessments of Temperature Maintenance Using In Vitro Culture Techniques
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Purpose : To assess the ability of various facets of embryo culture (microscope stage warmers, volumes of culture media, culture vessel lids, and type of culture incubator) to maintain a constant temperature in vitro.
Methods : Ability to maintain 37.0°C in the microenvironment of gametes was recorded by digital thermocouple in the chosen facets of in vitro culture.
Results : Stage warmers are highly variable in their ability to maintain the set temperature (range 33.8°C–37.0°C after 60 s). Temperature loss in culture media is both volume and vessel dependent, and the direct heat transfer culture incubator (MINC) has superior temperature maintenance compared with a large volume air convection incubator (FORMA), where temperature regain from 35.0°C to 37.0°C took 5.5 min compared to >20 min.
Conclusions : There are large measurable differences in the ability to maintain set temperature that depend on the stage warmer used, volume of media, use of vessel lids, and the type of incubator chosen for IVF culture.
- Shi DS, Avery B, Greve T: Effects of temperature gradients on in vitro maturation of bovine oocytes. Theriogenology 1998;50(4):667-674
- Rivera RM, Hansen PJ: Development of cultured bovine embryos after exposure to high temperatures in the physiological range. Reproduction 2001;121(1):107-115
- Pickering SJ, Johnson MH, Braude PR, Houliston E: Cytoskeletal organisation in fresh, aged and spontaneously activated human oocytes. Hum Reprod 1988;3:978-989
- Wang WH, Meng L, Hackett RJ, Odenbourg R, Keefe DL: Limited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light microscopy. Hum Reprod 2001;16(11):2374-2378
- Grinsted J, Kjer JJ, Blendstrup K, Pedersen JF: Is low temperature of the follicular fluid prior to ovulation necessary for normal oocyte development? Fertil Steril 1985;43(1):34-39
- Jurisicova A, Varmuza S, Casper RF: Programmed cell death and human embryo fragmentation. Mol Hum Reprod 1996;2:93-98
- Levy R, Benchaib M, Cordonier H, Guerin JF: Apoptosis in the pre-implantation embryo. Contracept Fertil Sex 1998;26:536-541
- Brison DR, Schultz RM: Apoptosis during mouse blastocyst formation: Evidence for a role for survival factors including TGF-a. Biol Reprod 1997;56:1088-1096
- Munne S, Magli C, Adler A, Wright G, deBoer K, Mortimer D, Tucker M, Cohen J, Aianaroli L: Treatment-related chromosome abnormalities in human embryos. Hum Reprod 1997;12:780-784
- Liebermann J, Graham J, Han T, Carter J, Tucker M: Oral 12: Temperature fluctuation of microdroplet in IVF. In Third Biennial Alpha Conference, 8-11 Sept. 2001, New York City, New York; Abstract book
- Objective Assessments of Temperature Maintenance Using In Vitro Culture Techniques
Journal of Assisted Reproduction and Genetics
Volume 19, Issue 8 , pp 368-375
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- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
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- Culture volume
- oil overlay
- stage warmers
- Industry Sectors
- Author Affiliations
- 1. City West IVF, 30 Mons Road, Westmead, New South Wales, 2145, Australia
- 2. City West IVF, 12 Caroline Street, Westmead, New South Wales, 2145, Australia
- 3. College of Social and Health Sciences, University of Western Sydney, Campbelltown Campus, New South Wales, Australia