World Journal of Microbiology and Biotechnology

, Volume 18, Issue 6, pp 559–561

Ethanol production from paper material using a simultaneous saccharification and fermentation system in a fed-batch basis


  • M. Ballesteros
  • J.M. Oliva
  • P. Manzanares
  • M.J. Negro
  • I. Ballesteros

DOI: 10.1023/A:1016378326762

Cite this article as:
Ballesteros, M., Oliva, J., Manzanares, P. et al. World Journal of Microbiology and Biotechnology (2002) 18: 559. doi:10.1023/A:1016378326762


In this work, a recycled paper-derived feedstock was used to produce ethanol by the simultaneous saccharification and fermentation (SSF) process using the thermotolerant yeast Kluyveromyces marxianus CECT 10875. At standard SSF conditions, the highest yield (about 80% of theoretical) was obtained at low substrate concentration and high enzyme loading. With increasing substrate concentration, mixing difficulties appeared which prevented an adequate SSF process performance and limited ethanol production. An SSF fed-batch procedure was then used which permitted an increase in substrate concentrations while maintaining SSF yields similar to that obtained at standard SSF, thus allowing an increased final ethanol production (about 18 g/l).

Ethanolpaper-derived materialssimultaneous saccharification and fermentation

Copyright information

© Kluwer Academic Publishers 2002