Salivary fluid secretion in the ixodid tick Rhipicephalus appendiculatus is inhibited by Thogoto virus infection
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Adult Rhipicephalus appendiculatus ticks, infectedwith Thogoto (THO) virus or control, were fed on guinea pigs and removed atintervals throughout the feeding cycle. Salivary fluid secretion was measuredbyan in vitro technique. The salivary glandsof infected, partially-fed ticks secreted fluid in vitro at about 75% the rateof controls, but the difference between infected and controls among engorgedticks was not statistically significant. Basal and DA-stimulated levels ofcyclic AMP (cAMP) were determined in isolated glands and were significantlyaffected by THO virus infection. The differences in secretory rate amongcontroland infected ticks could not be explained in terms of altered cAMP levels.Haemolymph volume was measured by a tracer-dilution technique using3H-inulin. The mean haemolymph volume for both THO-infected andcontrol groups was between 23–24% body weight throughout the feedingcycle, indicating that infection by this arbovirus did not influence salivaryfluid secretion via altered haemolymph volume. The mechanism by which THO virusaffects secretory activity of its tick vector remains unknown.
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- Salivary fluid secretion in the ixodid tick Rhipicephalus appendiculatus is inhibited by Thogoto virus infection
Experimental & Applied Acarology
Volume 25, Issue 8 , pp 661-674
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- Cyclic AMP
- Rhipicephalus appendiculatus
- Thogoto virus
- Tick salivary glands
- Industry Sectors
- Author Affiliations
- 1. Department of Biological Sciences, University of Alberta, T6G 2E9, Edmonton, Canada
- 2. Department of Zoology, University of Aberdeen, Tilleydrone Avenue, AB24 2TZ, Aberdeen, UK
- 3. Institute of Virology and Environmental Microbiology, Mansfield Road, OX1 3SR, Oxford, UK