Pharmaceutical Research

, Volume 13, Issue 4, pp 547–552

Response Surface Method: A Novel Strategy to Optimize lontophoretic Transdermal Delivery of Thyrotropin-releasing Hormone

  • Yi-You Huang
  • Shian-Min Wu
  • Cheng-Yi Wang
Article

DOI: 10.1023/A:1016089819967

Cite this article as:
Huang, Y., Wu, S. & Wang, C. Pharm Res (1996) 13: 547. doi:10.1023/A:1016089819967

Abstract

Purpose. To maximize the iontophoretic transdermal delivery rate of thyrotropin-releasing hormone (TRH) facilitated by periodically monophase-pulsed current across excised skin.

Methods. The pH of the buffer, the ionic strength in the solution, the frequency of the periodically monophase-pulsed current and the current on/off ratio were chosen as the key variables. A response surface method was applied to optimize the transdermal delivery rate of TRH under different operational conditions.

Results. The optimum operating conditions were achieved via experimentation based on the response surface method by systematically adjusting the pH of the buffer, the ionic strength in the solution, the current amplitude, frequency and the active temporal ratio of the pulsed current. The rate of permeation of TRH crossing the skin during iontophoresis varied from two to ten-fold, depending on operating conditions.

Conclusions. Only a few steps, two in this work, were needed to reach the optimal. The response surface near the region of the maximal point was thoroughly described with a quadratic function. A maximal transdermal rate of permeation of TRH, 103.2 µg h−1 cm−2, was obtained when the donor solution was at pH = 7.0, ionic strength = 0.037, and with a periodically monophase-pulsed current iontophoresis with duty cycle = 75%. The effect of pulse frequency was not statistically significant.

optimizationiontophoretic transdermal deliverythyrotropin releasing hormone (TRH)response surface method

Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Yi-You Huang
    • 1
  • Shian-Min Wu
    • 2
  • Cheng-Yi Wang
    • 3
  1. 1.Center for Biomedical Engineering, College of MedicineNational Taiwan UniversityTaipeiTaiwan
  2. 2.Department of Chemical EngineeingTunghai UniversityTai-chungTaiwan
  3. 3.Department of Internal Medicine, College of MedicineNational Taiwan UniversityTaipeiTaiwan, Republic of China