A Sex-linked Microsatellite Locus Isolated from the Y Chromosome of Lake Charr, Salvelinus Namaycush
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A small-insert library was constructed after microdissection of the short arm of the Y chromosome of lake charr, Salvelinus namaycush. Clones from this library were sequenced and two dinucleotide CA-repeat microsatellite sequences were recovered. Oligonucleotide primers for one locus were designed and optimized for amplification by PCR. This locus (designated Yp136) was tested for sex linkage in twelve lake charr families and found to be a distance of 37 centimorgans (cM) from the sex-determining locus. This microsatellite locus was also examined in three informative, gynogenetic/diploid, lake charr crosses for linkage to the centromere on the X chromosome. Results from these families show that this locus is 19 cM from the centromere. The combination of linkage data and microdissection information places the sex-determining locus near the telomere of the Y chromosome in lake charr. This is consistent with studies of several other fish species including some salmonids that place the sex locus near the telomere.
- Allendorf, F.W. & G.H. Thorgaard. 1984. Tetraploidy and the evolution of salmonid fishes. pp. 1–53. In: J.B. Turner (ed.) Evolutionary Genetics of Fishes, Plenum Publishing, New York.
- Allendorf, F.W., W.A. Gellman & G.H. Thorgaard. 1994. Sex-linkage of two enzyme loci in rainbow trout. Heredity 72: 498–507.
- Bull, J.J. 1983. Evolution of sex determining mechanisms. Benjamin Cummings, Menlo Park. 316 pp.
- Bull, J.J. 1989. Evolution and variety of sex-determining mechanisms in amniote vertebrates. pp. 57–65. In: S.S. Wachtel (ed.) Evolutionary Mechanisms in Sex Determination, CRC Press Inc., Boca Raton.
- Carrasco, L.A.P., D.J. Penman & N. Bromage. 1999. Evidence for the presence of sex chromosomes in the Nile tilapia (Oreochromis niloticus) from synaptonemal complex analysis of XX, XY and YY genotypes. Aquaculture 173: 207–218.
- Devlin, R.H., G.W. Stone & D.E. Smailus. 1998. Extensive directtandem organization of a long repeat DNA sequence on the Y chromosome of chinook salmon (Oncorhynchus tshawytscha). J. Mol. Evol. 46: 277–287.
- Devlin, R.H., B.K. McNeil, T.D.D. Groves & E.M. Donaldson. 1991. Isolation of Y-chromosomal DNA probe capable of determining genetic sex in chinook salmon (Oncorhynchus tshawytscha). Can. J. Fish. Aquat. Sci. 48: 1606–1612.
- Donaldson, E.M. & G.A. Hunter. 1982. Sex control in fish with particular reference to salmonids. Can. J. Fish. Aquat. Sci. 39: 99–110.
- Du, S.J., R.H. Devlin & C.L. Hew. 1993. Genomic structure of growth hormone genes in chinook salmon (Oncorhynchus tshawytscha): presence of two functional genes, GH-I and GH-II, and a male-specific pseudogene, GH-Ψ. DNA Cell Bio. 12: 739–751.
- Ellis, N.A. 1996. Humansex chromosome evolution. pp. 229–260. In: M. Jackson, T. Strachan & G. Dover (ed.) Human Genome Evolution, BIOS Scientific Publishers, Oxford.
- Forbes, S.H., K.L. Knudson, T.W. North & F.W. Allendorf. 1994. One of two growth hormone genes in coho salmon is sexlinked. Proceedings of the National Academy of Sciences 91: 1628–1631.
- Gallardo, M.H., J.W. Bickham, R.L. Honeycutt, R.A. Ojeda & N. Kohler. 1999. Discovery of tetraploidy in a mammal. Nature 401: 341.
- Graves, J.A.M. & K.C. Reed. 1993. Organization, function and evolution of sex chromosomes. pp. 129–135. In: K.C. Reed & J.A.M. Graves (ed.) Sex Chromosomes and Sex-determining Genes, Harwood Academic Publishers, Chur.
- Hunter, G.A., E.M. Donaldson, J. Stoss & I. Baker. 1983. Production of monosex female groups of chinook salmon (Oncorhynchus tshawytscha) by the fertilization of normal ova with sperm from sex-reversed females. Aquaculture 33: 355–364.
- Jegalian, K. & D.C. Page. 1998. A proposed path by which genes common to mammalian X and Y chromosomes evolve to become X inactivated. Nature 394: 776–780.
- Matsuda, M., C. Matsuda, S. Hamaguchi & M. Sakaizumi. 1998. Identification of the sex chromosomes of the medaka, Oryzias latipes, by fluorescence in situ hybridization. Cytogenet. Cell. Genet. 82: 257–262.
- Nanda, I., Z. Shan, M. Scharl, D.W. Burt, M. Koehler, H. Nothwang, F. Grutzner, I.R. Paton, D. Windsor, I. Dunn, W. Engel, P. Staeheli, S. Mizuno, T. Haaf & M. Schmid. 1999. 300 million years of conserved synteny between chicken Z and human chromosome 9. Nat. Genet. 21: 258–259.
- Parsons, J.E & G.H. Thorgaard. 1985. Production of androgenetic diploid rainbow trout. J. Hered. 76: 177–181.
- Phillips, R.B. & P.E. Ihssen. 1985. Identification of sex chromosomes in lake trout (Salvelinus namaycush). Cytogenet. Cell. Genet. 39: 14–18.
- Reed, K.M., S.K. Bohlander & R.B. Phillips. 1995. Microdissection of the Y chromosome and fluorescence in situ hybridization analysis of the sex chromosomes of lake trout, Salvelinus namaycush. Chromosome Res. 3: 221–226.
- Reed, K.M. & R.B. Phillips. 1995. Molecular characterization and cytogenetic analysis of highly repetitive DNAs of lake trout, Salvelinus namaycush. Chromosoma 104: 242–251.
- Rice, W.R. 1994. Degeneration of a nonrecombining chromosome. Science 263: 230–232.
- Sakomoto, T., R.G. Danzmann, K. Gharbi, P. Howard, A. Ozaki, S.K. Khoo, R.A. Woram, N. Okamoto, M.M. Ferguson, L. Holm, R. Guyomard & H. Bjorn. 2000. A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates. Genetics 155: 1331–1345.
- Thorgaard, G. H. 1977. Heteromorphic sex chromosomes in male rainbow trout. Science 196: 900–902.
- Thorgaard, G.H., F.W. Allendorf & K.L. Knudsen. 1983. Gene centromere mapping in rainbow trout: high interference over long map distances. Genetics 103: 771–783.
- Traut, W. 1999. The evolution of sex chromosomes in insects: differentiation of sex chromosomes in flies and moths. Eur. J. Entomol. 96: 227–235.
- A Sex-linked Microsatellite Locus Isolated from the Y Chromosome of Lake Charr, Salvelinus Namaycush
Environmental Biology of Fishes
Volume 64, Issue 1-3 , pp 211-216
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- 1. Department of Biological Sciences, University of Wisconsin-Milwaukee, PO Box 413, Milwaukee, WI, 53201, U.S.A
- 2. Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, Veterinary Science Building, 1971 Commonwealth Avenue, St. Paul, MN, 55108, U.S.A
- 3. Ontario Ministry of Natural Resources, Trent University, Peterborough, Ontario, K9J 8N8, Canada
- 4. School of Biological Sciences, Washington State University, 14204 NE Salmon Creek Ave., Vancouver, WA, 98686-9600, U.S.A.