Journal of Clinical Immunology

, Volume 22, Issue 4, pp 195–205

Age-Related Decrease in the Inducibility of Heat-Shock Protein 70 in Human Peripheral Blood Mononuclear Cells

  • R. Njemini
  • M. Vanden Abeele
  • C. Demanet
  • M. Lambert
  • S. Vandebosch
  • T. Mets
Article

DOI: 10.1023/A:1016036724386

Cite this article as:
Njemini, R., Abeele, M.V., Demanet, C. et al. J Clin Immunol (2002) 22: 195. doi:10.1023/A:1016036724386

Abstract

We have investigated the effect of age and of the presence of proinflammatory cytokines on Hsp 70 production in human peripheral blood mononuclear cells, using flow cytometry. Twenty-seven women and 23 men, all apparently healthy, participated in the study. At 37°C, the percentage of Hsp 70-producing monocytes and lymphocytes, as well as the level of Hsp 70 in monocytes, were negatively influenced by age. After exposure of the cells to 42°C, the increase of Hsp 70 production was more pronounced in monocytes than in lymphocytes; both the intensity of Hsp 70 production and the percentage of Hsp 70-producing cells were negatively influenced by the age of the subjects, as well for monocytes as for lymphocytes. There was a negative correlation between the intensity of Hsp 70 production by monocytes exposed to 42°C and the serum levels of tumor necrosis factor-α and interleukin-6. In conclusion, in human monocytes and lymphocytes, heat-induced Hsp 70 production is reduced with increasing age and is negatively influenced in monocytes by proinflammatory cytokines.

Peripheral mononuclear blood cellsheat shockheat-shock protein 70agingproinflammatory cytokines

Copyright information

© Plenum Publishing Corporation 2002

Authors and Affiliations

  • R. Njemini
    • 1
  • M. Vanden Abeele
    • 1
  • C. Demanet
    • 2
  • M. Lambert
    • 1
  • S. Vandebosch
    • 1
  • T. Mets
    • 1
  1. 1.Geriatric Unit, Academic HospitalFree University Brussels (VUB)Belgium
  2. 2.HLA and Immunology Laboratory, Academic HospitalFree University Brussels (VUB)Belgium