Article

Biochemistry (Moscow)

, Volume 67, Issue 5, pp 525-533

First online:

Kinetics of Thermal Aggregation of Tobacco Mosaic Virus Coat Protein

  • B. I. KurganovAffiliated withBach Institute of Biochemistry, Russian Academy of Sciences Email author 
  • , E. R. RafikovaAffiliated withBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
  • , E. N. DobrovAffiliated withBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University

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Abstract

The kinetics of thermal aggregation of coat protein (CP) of tobacco mosaic virus (TMV) have been studied at 42 and 52°C in a wide range of protein concentrations, [P]0. The kinetics of aggregation were followed by monitoring the increase in the apparent absorbance (A) at 320 nm. At 52°C the kinetic curves may be approximated by the exponential law in the range of TMV CP concentrations from 0.02 to 0.30 mg/ml, the first order rate constant being linearly proportional to [P]0 (50 mM phosphate buffer, pH 8.0). The analogous picture was observed at 42°C in the range of TMV CP concentrations from 0.01 to 0.04 mg/ml (100 mM phosphate buffer, pH 8.0). At higher TMV CP concentrations the time of half-conversion approaches a limiting value with increasing [P]0 and at sufficiently high protein concentrations the kinetic curves fall on a common curve in the coordinates {A/A lim; t} (t is time and A lim is the limiting value of A at t → ∞). According to a mechanism of aggregation of TMV CP proposed by the authors at rather low protein concentrations the rate of aggregation is limited by the stage of growth of aggregate, which proceeds as a reaction of the pseudo-first order, whereas at rather high protein concentrations the rate-limiting stage is the stage of protein molecule unfolding.

tobacco mosaic virus coat protein thermal aggregation kinetics