Inflammation

, Volume 26, Issue 3, pp 103–110

Human Umbilical Vein Endothelial Cells Express Complement Receptor 1 (CD35) and Complement Receptor 4 (CD11c/CD18) in vitro

  • Harald Langeggen
  • Knut Erik Berge
  • Egil Johnson
  • Geir Hetland
Article

DOI: 10.1023/A:1015585530204

Cite this article as:
Langeggen, H., Berge, K.E., Johnson, E. et al. Inflammation (2002) 26: 103. doi:10.1023/A:1015585530204

Abstract

We have examined complement receptors on human umbilical vein endothelial cells (HUVEC) and found that they express complement receptor 1 (CR1, CD35) and complement receptor 4 (CR4, CD11c/CD18), but not complement receptor 3 (CR3, CD11b/CD18). Binding of monoclonal antibodies against CR1 (CD35) and CR4 (CD11c/CD18) to HUVEC was demonstrated by flow cytometry. The presence of the corresponding mRNAs was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequencing of the amplified cDNA fragments. When HUVEC were treated with inflammatory mediators, chemotactic agents or the secretagogue phorbol-12-myristate-13-acetate (PMA), no change in reactivity to CR1 (CD35) or CR4 (CD11c/CD18) monoclonal antibodies was detected on the surface of the cells compared with untreated cells. The presence of CR1 (CD35) and CR4 (CD11c/CD18) on HUVEC indicates that endothelial cells (EC) have the potential to bind C3b and iC3b, respectively, which both mediate biological effects in the course of complement activation.

CR1 (CD35) CR4 (CD11c/CD18) endothelial cells 

Copyright information

© Plenum Publishing Corporation 2002

Authors and Affiliations

  • Harald Langeggen
    • 1
    • 2
  • Knut Erik Berge
    • 3
  • Egil Johnson
    • 1
    • 4
  • Geir Hetland
    • 5
  1. 1.Ullevål HospitalUniversity of OsloOsloNorway
  2. 2.Department of Pediatric Surgery, Ullevål HospitalUniversity of OsloOsloNorway
  3. 3.Institute of Medical Genetics, Ullevål HospitalUniversity of OsloOsloNorway
  4. 4.Department of Gastroenterological Surgery, Ullevål HospitalUniversity of OsloOsloNorway
  5. 5.Department of Environmental MedicineNational Institute of Public HealthOsloNorway

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