Molecular and Cellular Biochemistry

, Volume 233, Issue 1, pp 1–8

N-cadherin involvement in the heterotypic adherence of malignant T-cells to epithelia

  • Authors
  • Irwan T. Makagiansar
  • Helena Yusuf-Makagiansar
  • Atsutoshi Ikesue
  • Anna M. Calcagno
  • Joseph S. Murray
  • Teruna J. Siahaan
Article

DOI: 10.1023/A:1015556625038

Cite this article as:
Makagiansar, I.T., Yusuf-Makagiansar, H., Ikesue, A. et al. Mol Cell Biochem (2002) 233: 1. doi:10.1023/A:1015556625038
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Abstract

N-cadherin, a cell adhesion molecule normally found in neural cell tissue, has been found recently to be expressed on the surface of malignant T-cells. The function of N-cadherin on these cells remains unclear. Heterotypic assays between Molt-3 T lymphoblastic leukemia cells and Caco-2 epithelial monolayers were examined under different conditions to assess the functional role of N-cadherin. The results indicate that adherence of Molt-3 cells to Caco-2 monolayers was reduced significantly following pretreatment of Molt-3 cells with 100 μM of an N-cadherin-derived antagonist decapeptide. In contrast, pretreatment of Molt-3 cells with an anti-N-cadherin antibody raised against the first 20 amino acids of N-cadherin sequence led to a surprisingly marked enhancement of Molt-3 cell adherence to Caco-2 monolayers. In addition, the presence of anti-N-cadherin antibody neutralized the inhibitory effect of anti-ICAM-1 on Molt-3 adhesion to Caco-2 monolayers. This novel finding demonstrates that external stimulus through the N-cadherin amino terminus can modulate adhesion of malignant T-cells to epithelia and may promote their ability to invade or metastasize to inflammatory sites.

malignant T-cellsMolt-3Caco-2N-cadherinβ-cateninanti-N-cadherin antibodyanti-ICAM-1 antibody

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© Kluwer Academic Publishers 2002