Biometals

, Volume 15, Issue 2, pp 103–120

Metal chelating properties of pyridine-2,6-bis(thiocarboxylic acid) produced by Pseudomonas spp. and the biological activities of the formed complexes

  • Marc S. Cortese
  • Andrzej Paszczynski
  • Thomas A. Lewis
  • Jonathan L. Sebat
  • Vladimir Borek
  • Ronald L. Crawford
Article

DOI: 10.1023/A:1015241925322

Cite this article as:
Cortese, M.S., Paszczynski, A., Lewis, T.A. et al. Biometals (2002) 15: 103. doi:10.1023/A:1015241925322

Abstract

We evaluated the ability of pyridine-2,6-bis(thiocarboxylic acid) (pdtc) to form complexes with 19 metals and 3 metalloids. Pdtc formed complexes with 14 of the metals. Two of these metal:pdtc complexes, Co:(pdtc)2 and Cu:pdtc, showed the ability to cycle between redox states, bringing to 4 the number of known redox-active pdtc complexes. A precipitant formed when pdtc was added to solutions of As, Cd, Hg, Mn, Pb, and Se. Additionally, 14 of 16 microbial strains tested were protected from Hg toxicity when pdtc was present. Pdtc also mediated protection from the toxic effects of Cd and Te, but for fewer strains. Pdtc by itself does not facilitate iron uptake, but increases the overall level of iron uptake of Pseudomonas stutzeri strain KC and P. putida DSM301. Both these pseudomonads could reduce amorphous Fe(III) oxyhydroxide in culture. In vitro reactions showed that copper and pdtc were required for this activity. This reaction may derive its reducing power from the hydrolysis of the thiocarboxyl groups of pdtc.

Pseudomonas stutzerithiocarboxylate2,6-pyridinedicarbothioic acidsecondary metabolitemetal chelation

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Marc S. Cortese
    • 1
  • Andrzej Paszczynski
    • 1
  • Thomas A. Lewis
    • 2
  • Jonathan L. Sebat
    • 1
  • Vladimir Borek
    • 3
  • Ronald L. Crawford
    • 1
  1. 1.Environmental Biotechnology InstituteUniversity of IdahoMoscowUSA
  2. 2.Department of Microbiology and Molecular GeneticsUniversity of VermontBurlingtonUSA
  3. 3.Department of Plant, Soil, and Entomological SciencesUniversity of IdahoMoscowUSA