Molecular Breeding

, Volume 8, Issue 4, pp 317–322

Field testing of transgenic rapeseed cv. Hero transformed with a yeast sn-2 acyltransferase results in increased oil content, erucic acid content and seed yield

Authors

  • David C. Taylor
    • National Research Council of CanadaPlant Biotechnology Institute
  • Vesna Katavic
    • Saskatchewan Wheat Pool Agricultural Research and Development
  • Jitao Zou
    • National Research Council of CanadaPlant Biotechnology Institute
  • Samuel L. MacKenzie
    • National Research Council of CanadaPlant Biotechnology Institute
  • Wilfred A. Keller
    • National Research Council of CanadaPlant Biotechnology Institute
  • Jing An
    • National Research Council of CanadaPlant Biotechnology Institute
  • Winnie Friesen
    • National Research Council of CanadaPlant Biotechnology Institute
  • Dennis L. Barton
    • National Research Council of CanadaPlant Biotechnology Institute
  • Kalie K. Pedersen
    • National Research Council of CanadaPlant Biotechnology Institute
  • E. Michael Giblin
    • National Research Council of CanadaPlant Biotechnology Institute
  • Yan Ge
    • National Research Council of CanadaPlant Biotechnology Institute
  • Melanie Dauk
    • National Research Council of CanadaPlant Biotechnology Institute
  • Cory Sonntag
    • Saskatchewan Wheat Pool Agricultural Research and Development
  • Tammy Luciw
    • Saskatchewan Wheat Pool Agricultural Research and Development
  • Daryl Males
    • Saskatchewan Wheat Pool Agricultural Research and Development
Article

DOI: 10.1023/A:1015234401080

Cite this article as:
Taylor, D.C., Katavic, V., Zou, J. et al. Molecular Breeding (2002) 8: 317. doi:10.1023/A:1015234401080

Abstract

A major goal of our research is to produce, by genetic manipulation, Brassica napus L. cultivars with higher levels of 22:1 in their seed oil than in present Canadian HEA cultivars developed through traditional breeding. Previously, we reported that transgenic expression of a mutated yeast sn-2 acyltransferase (SLC1-1) in industrial rapeseed cv. Hero resulted in increased seed oil content, increased proportions of erucic acid and increased average seed weight (Zou et al. 1997). Those results were reported only for plants grown in a controlled greenhouse setting. Here we report a summary of the results from two successive years of field trials with T4 and T5 generations of B. napus cv. Hero transformed with the SLC1-1 gene. These trials, conducted at Rosthern, Saskatchewan, in two very different growing seasons, show that the SLC1-1 transgenics clearly and consistently out-performed controls, with much increased oil and 22:1 contents, as well as yield, under varying field conditions.

Brassica napusErucic acidOil contentRapeseedSeed weightYeast acyltransferase

Copyright information

© Kluwer Academic Publishers 2002