Breast Cancer Research and Treatment

, Volume 73, Issue 1, pp 75–83

Effect of Selective Ablation of Proliferating Mammary Epithelial Cells on MNU Induced Rat Mammary Tumorigenesis

Authors

  • Lakshmi Sivaraman
    • Department of Molecular and Cellular BiologyBaylor College of Medicine
  • Jason Gay
    • Department of Molecular and Cellular BiologyBaylor College of Medicine
  • Susan G Hilsenbeck
    • Breast CenterBaylor College of Medicine
  • H David Shine
    • Department of Molecular and Cellular Biology
    • Department of Neurosurgery
    • Division of Neuroscience
    • Center for Cell and Gene TherapyBaylor College of Medicine
  • Orla M Conneely
    • Department of Molecular and Cellular BiologyBaylor College of Medicine
  • Daniel Medina
    • Department of Molecular and Cellular BiologyBaylor College of Medicine
  • Bert W O'Malley
    • Department of Molecular and Cellular BiologyBaylor College of Medicine
Article

DOI: 10.1023/A:1015227719105

Cite this article as:
Sivaraman, L., Gay, J., Hilsenbeck, S.G. et al. Breast Cancer Res Treat (2002) 73: 75. doi:10.1023/A:1015227719105

Abstract

Proliferating cells within the terminal end buds of the virgin female rat mammary gland are the most susceptible to chemical carcinogen induced tumorigenesis. We hypothesized that selective ablation of proliferating cells in the mammary gland would reduce mammary tumor incidence upon carcinogen challenge. Selective ablation of proliferating cells was achieved by intraductal injections of Adv-RSV-tk and gancyclovir administration. Despite efficient viral transduction of the thymidine kinase protein and the apparent elimination of >90% of the prolif-erating cells, the rats exhibited a higher incidence of MNU induced mammary tumors arising with shorter latency as compared to control animals. Several possible explanations of the puzzling relationship between elimination of cycling cells and increased tumor incidence are discussed and alternative strategies for the prevention of breast cancer are proposed.

Adv-RSV-tk/GCVcarcinogenesismammary glandMNUproliferating cells

Copyright information

© Kluwer Academic Publishers 2002