World Journal of Microbiology and Biotechnology

, Volume 18, Issue 4, pp 303-308

First online:

Optimization of dilution rate for the production of value added product and simultaneous reduction of organic load from pineapple cannery waste

  • J.N. NigamAffiliated withBiochemistry Division, Regional Research Laboratory, Jorhat
  • , M.C. KakatiAffiliated withComputer Section, Regional Research Laboratory, Jorhat

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Candida utiilis NRRL Y-900 was grown on pineapple cannery waste as the sole carbon and energy source in a chemostat at dilution rates ranging between 0.05 and 0.65 h−1 to determine the growth kinetics. The cell yield coefficient varied with dilution rate and a maximum value of 0.662 ± 0.002 gx/gcarb was obtained at a dilution rate of 0.4 h−1. At steady state, the concentrations of carbohydrate, reducing sugar, and chemical oxygen demand (COD) appeared to follow Monod kinetics. At maximum specific growth rate (μmax) 0.65 h−1, the saturation constants for carbohydrate, reducing sugar and COD were 0.51 ± 0.02 gcarb/1, 0.046 ± 0.003 grs/1, and 1.036 ± 0.001 gCOD/1, respectively. Maximum biomass productivity (Q x max) 2.8 ± 0.03 gx/1 h was obtained at a dilution rate of 0.5 h−1. At this dilution rate, only 71.0 ± 0.41% COD was removed whereas at a dilution rate of 0.1 h−1, 98.2 ± 0.35% reduction in COD was achieved. At a dilution rate of 0.4 h−1, the optimal yeast productivity and reduction in COD were 2.7 ± 0.13 gp/1 h, and 84.2 ± 0.42%, respectively.

Biomass Candida utilis chemostat continuous culture growth kinetics pineapple cannery waste steady state