Biologia Plantarum

, Volume 45, Issue 1, pp 91–97

Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane

  • R.F. Fornazier
  • R.R. Ferreira
  • A.P. Vitória
  • S.M.G. Molina
  • P.J. Lea
  • R.A. Azevedo
Article

DOI: 10.1023/A:1015100624229

Cite this article as:
Fornazier, R., Ferreira, R., Vitória, A. et al. Biologia Plantarum (2002) 45: 91. doi:10.1023/A:1015100624229

Abstract

Sugar cane (Saccharum officinarum L. cv. Copersucar SP80-3280) seedlings were grown in nutrient solution with varying concentrations (0, 2 and 5 mM) of cadmium chloride for 96 h. Leaves were analysed for catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) activities. Although a clear effect of CdCl2 on plant growth was observed, the activity of SOD was not altered significantly. However, the CAT activity decreased as the concentration of CdCl2 increased. GR exhibits a significant increase in activity at 2 and 5 mM CdCl2. CAT and SOD isoenzymes were further characterised by analysis in non-denaturing PAGE. Activity staining for SOD revealed up to seven isoenzymes in untreated control and 2 mM CdCl2 treated plants, corresponding to Cu/Zn-SOD isoenzymes. At 5 mM CdCl2, only six Cu/Zn-SOD isoenzymes were observed. No Fe-SOD and Mn-SOD isoenzymes were detected. For CAT, one band of activity was observed.

catalaseglutathione reductaseheavy metalsphytochelatinsreactive oxygen speciesSaccharum officinarumsuperoxide dismutase

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • R.F. Fornazier
    • 1
  • R.R. Ferreira
    • 1
  • A.P. Vitória
    • 2
  • S.M.G. Molina
    • 1
  • P.J. Lea
    • 3
  • R.A. Azevedo
    • 1
  1. 1.Departamento de Genética, Escola Superior de Agricultura Luiz de QueirozUniversidade de São PauloPiracicaba CEPBrazil
  2. 2.Departamento de Ciências BiológicasUniversidade Estadual de Feira de SantanaFeira de Santana CEPBrazil
  3. 3.Department of Biological SciencesUniversity of LancasterLancasterUK