Development of a responsive methanol sensor and its application in Pichia pastoris fermentation
- Cite this article as:
- Zhou, XS., Lu, J., Fan, WM. et al. Biotechnology Letters (2002) 24: 643. doi:10.1023/A:1015047425493
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A methanol sensor was developed with response time less than 2 min. It was unaffected by the dissolved O2 concentration, agitation speed or pH value. When the sensor was used to monitor the methanol concentration on-line during hirudin production by recombinant Pichia pastoris, the cell dry weight was up to 155 g l−1, and hirudin was 1.4 g l−1.