The Histochemical Journal

, Volume 33, Issue 9, pp 531–535

DNase I Is Present in the Chief Cells of Human and Rat Stomachs

Authors

  • S. Tsutsumi
    • First Department of SurgeryGunma University School of Medicine, Maebashi
  • Y. Kaneko
    • First Department of SurgeryGunma University School of Medicine, Maebashi
  • T. Asao
    • First Department of SurgeryGunma University School of Medicine, Maebashi
  • H. Kuwano
    • First Department of SurgeryGunma University School of Medicine, Maebashi
  • S. Kudo
    • Department of Anatomy IGunma University School of Medicine, Maebashi
  • H. Takeshita
    • Department of Legal MedicineGunma University School of Medicine, Maebashi
  • T. Yasuda
    • Department of BiologyFukui Medical University, Matsuoka
  • K. Kishi
    • Department of Legal MedicineGunma University School of Medicine, Maebashi
Article

DOI: 10.1023/A:1014999624430

Cite this article as:
Tsutsumi, S., Kaneko, Y., Asao, T. et al. Histochem J (2001) 33: 531. doi:10.1023/A:1014999624430

Abstract

The distribution of deoxyribonuclease I (DNase I) in human and rat stomachs was examined by biochemical, molecular biological and immunohistochemical techniques. By the use of monoclonal anti-human DNase I and polyclonal anti-rat DNase I antibodies, we determined that strong immunoreactivity was present in the cytoplasm of chief cells of the human fundus and the rat pars glandularis, respectively. High DNase I enzyme activity was detected in tissue homogenates of both human fundus and rat pars glandularis. The presence of DNase I-specific mRNA was verified by reverse transcriptase-polymerase chain reaction analysis of the total RNAs extracted from human and rat stomachs. Immunoelectron microscopy revealed gold particles localized in the chief cells, with most labelling in exocrine secretory granules. These results show that the chief cells of human and rat stomach produce DNase I. This is the first report to demonstrate that secretion of DNase I is controlled by the chief cells in human and rat stomachs.

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© Kluwer Academic Publishers 2001