The Histochemical Journal

, Volume 33, Issue 9, pp 537–543

GABA Immunoreactivity in the Human Cerebellar Cortex: A Light and Electron Microscopical Study

  • Vincenzo Benagiano
  • Luisa Roncali
  • Daniela Virgintino
  • Paolo Flace
  • Mariella Errede
  • Anna Rizzi
  • Francesco Girolamo
  • David Robertson
  • Joachim Bormann
  • Glauco Ambrosi
Article

DOI: 10.1023/A:1014903908500

Cite this article as:
Benagiano, V., Roncali, L., Virgintino, D. et al. Histochem J (2001) 33: 537. doi:10.1023/A:1014903908500

Abstract

The distribution of γ-aminobutyric acid (GABA) in surgical samples of human cerebellar cortex was studied by light and electron microscope immunocytochemistry using a polyclonal antibody generated in rabbit against GABA coupled to bovine serum albumin with glutaraldehyde. Observations by light microscopy revealed immunostained neuronal bodies and processes as well as axon terminals in all layers of the cerebellar cortex. Perikarya of stellate, basket and Golgi neurons showed evident GABA immunoreactivity. In contrast, perikarya of Purkinje neurons appeared to be negative or weakly positive. Immunoreactive tracts of longitudinally- or obliquely-sectioned neuronal processes and punctate elements, corresponding to axon terminals or cross-sectioned neuronal processes, showed a layer-specific pattern of distribution and were seen on the surface of neuronal bodies, in the neuropil and at microvessel walls. Electron microscope observations mainly focussed on the analysis of GABA-labelled axon terminals and of their relationships with neurons and microvessels. GABA-labelled terminals contained gold particles associated with pleomorphic vesicles and mitochondria and established symmetric synapses with neuronal bodies and dendrites in all cortex layers. GABA-labelled terminals associated with capillaries were seen to contact the perivascular glial processes, basal lamina and endothelial cells and to establish synapses with subendothelial unlabelled axons.

To our Master, Professor Rodolfo Amprino, with our great admiration, gratefulness and affection, on the occasion of his ninetieth birthday.

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Vincenzo Benagiano
    • 1
    • 2
  • Luisa Roncali
    • 2
  • Daniela Virgintino
    • 2
  • Paolo Flace
    • 2
  • Mariella Errede
    • 2
  • Anna Rizzi
    • 2
  • Francesco Girolamo
    • 2
  • David Robertson
    • 3
  • Joachim Bormann
    • 4
  • Glauco Ambrosi
    • 5
  1. 1.Facoltà di Medicina e ChirurgiaUniversità di FoggiaItaly
  2. 2.Dipartimento di Anatomia Umana e IstologiaUniversità di BariItaly
  3. 3.Haddow LaboratoriesInstitute of Cancer ResearchSuttonUK
  4. 4.Lehrstul für ZellphysiologieRuhr-UniversitätBochumGermany
  5. 5.Dipartimento di Anatomia Umana e IstologiaUniversità di BariItaly

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