The Histochemical Journal

, Volume 33, Issue 9, pp 537–543

GABA Immunoreactivity in the Human Cerebellar Cortex: A Light and Electron Microscopical Study

Authors

  • Vincenzo Benagiano
    • Facoltà di Medicina e ChirurgiaUniversità di Foggia
    • Dipartimento di Anatomia Umana e IstologiaUniversità di Bari
  • Luisa Roncali
    • Dipartimento di Anatomia Umana e IstologiaUniversità di Bari
  • Daniela Virgintino
    • Dipartimento di Anatomia Umana e IstologiaUniversità di Bari
  • Paolo Flace
    • Dipartimento di Anatomia Umana e IstologiaUniversità di Bari
  • Mariella Errede
    • Dipartimento di Anatomia Umana e IstologiaUniversità di Bari
  • Anna Rizzi
    • Dipartimento di Anatomia Umana e IstologiaUniversità di Bari
  • Francesco Girolamo
    • Dipartimento di Anatomia Umana e IstologiaUniversità di Bari
  • David Robertson
    • Haddow LaboratoriesInstitute of Cancer Research
  • Joachim Bormann
    • Lehrstul für ZellphysiologieRuhr-Universität
    • Dipartimento di Anatomia Umana e IstologiaUniversità di Bari
Article

DOI: 10.1023/A:1014903908500

Cite this article as:
Benagiano, V., Roncali, L., Virgintino, D. et al. Histochem J (2001) 33: 537. doi:10.1023/A:1014903908500

Abstract

The distribution of γ-aminobutyric acid (GABA) in surgical samples of human cerebellar cortex was studied by light and electron microscope immunocytochemistry using a polyclonal antibody generated in rabbit against GABA coupled to bovine serum albumin with glutaraldehyde. Observations by light microscopy revealed immunostained neuronal bodies and processes as well as axon terminals in all layers of the cerebellar cortex. Perikarya of stellate, basket and Golgi neurons showed evident GABA immunoreactivity. In contrast, perikarya of Purkinje neurons appeared to be negative or weakly positive. Immunoreactive tracts of longitudinally- or obliquely-sectioned neuronal processes and punctate elements, corresponding to axon terminals or cross-sectioned neuronal processes, showed a layer-specific pattern of distribution and were seen on the surface of neuronal bodies, in the neuropil and at microvessel walls. Electron microscope observations mainly focussed on the analysis of GABA-labelled axon terminals and of their relationships with neurons and microvessels. GABA-labelled terminals contained gold particles associated with pleomorphic vesicles and mitochondria and established symmetric synapses with neuronal bodies and dendrites in all cortex layers. GABA-labelled terminals associated with capillaries were seen to contact the perivascular glial processes, basal lamina and endothelial cells and to establish synapses with subendothelial unlabelled axons.

To our Master, Professor Rodolfo Amprino, with our great admiration, gratefulness and affection, on the occasion of his ninetieth birthday.

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© Kluwer Academic Publishers 2001