Plant Molecular Biology

, Volume 48, Issue 5, pp 551–573

Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley

  • Z. Neslihan Ozturk
  • Valentina Talamé
  • Michael Deyholos
  • Christine B. Michalowski
  • David W. Galbraith
  • Nermin Gozukirmizi
  • Roberto Tuberosa
  • Hans J. Bohnert
Article

DOI: 10.1023/A:1014875215580

Cite this article as:
Ozturk, Z.N., Talamé, V., Deyholos, M. et al. Plant Mol Biol (2002) 48: 551. doi:10.1023/A:1014875215580

Abstract

Responses to drought and salinity in barley (Hordeum vulgare L. cv. Tokak) were monitored by microarray hybridization of 1463 DNA elements derived from cDNA libraries of 6 and 10 h drought-stressed plants. Functional identities indicated that many cDNAs in these libraries were associated with drought stress. About 38% of the transcripts were novel and functionally unknown. Hybridization experiments were analyzed for drought- and salinity-regulated sequences, with significant changes defined as a deviation from the control exceeding 2.5-fold. Responses of transcripts showed stress-dependent expression patterns and time courses. Nearly 15% of all transcripts were either up- or down-regulated under drought stress, while NaCl led to a change in 5% of the transcripts (24 h, 150 mM NaCl). Transcripts that showed significant up-regulation under drought stress are exemplified by jasmonate-responsive, metallothionein-like, late-embryogenesis-abundant (LEA) and ABA-responsive proteins. Most drastic down-regulation in a category was observed for photosynthesis-related functions. Up-regulation under both drought and salt stress was restricted to ESTs for metallothionein-like and LEA proteins, while increases in ubiquitin-related transcripts characterized salt stress. A number of functionally unknown transcripts from cDNA libraries of drought-stressed plants showed up-regulation by drought but down-regulation by salt stress, documenting how precisely transcript profiles report different growth conditions and environments.

drought stressHordeum vulgaremicroarray hybridizationsalinity stressstress-regulated transcripts

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Z. Neslihan Ozturk
    • 1
    • 2
  • Valentina Talamé
    • 1
    • 3
  • Michael Deyholos
    • 4
  • Christine B. Michalowski
    • 1
  • David W. Galbraith
    • 4
  • Nermin Gozukirmizi
    • 2
  • Roberto Tuberosa
    • 3
  • Hans J. Bohnert
    • 1
  1. 1.Department of Biochemistry and Molecular BiophysicsUniversity of ArizonaTucsonUSA
  2. 2.TUBITAK, Marmara Research CenterResearch Institute for Genetic EngineeringTurkey
  3. 3.Department of Agroenvironmental Science and TechnologyUniversity of BolognaBolognaItaly
  4. 4.Departments of Plant Biology and of Crop SciencesUniversity of IllinoisUrbanaUSA