Plant Molecular Biology

, Volume 48, Issue 5, pp 551–573

Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley

Authors

  • Z. Neslihan Ozturk
    • Department of Biochemistry and Molecular BiophysicsUniversity of Arizona
    • TUBITAK, Marmara Research CenterResearch Institute for Genetic Engineering
  • Valentina Talamé
    • Department of Biochemistry and Molecular BiophysicsUniversity of Arizona
    • Department of Agroenvironmental Science and TechnologyUniversity of Bologna
  • Michael Deyholos
    • Departments of Plant Biology and of Crop SciencesUniversity of Illinois
  • Christine B. Michalowski
    • Department of Biochemistry and Molecular BiophysicsUniversity of Arizona
  • David W. Galbraith
    • Departments of Plant Biology and of Crop SciencesUniversity of Illinois
  • Nermin Gozukirmizi
    • TUBITAK, Marmara Research CenterResearch Institute for Genetic Engineering
  • Roberto Tuberosa
    • Department of Agroenvironmental Science and TechnologyUniversity of Bologna
  • Hans J. Bohnert
    • Department of Biochemistry and Molecular BiophysicsUniversity of Arizona
Article

DOI: 10.1023/A:1014875215580

Cite this article as:
Ozturk, Z.N., Talamé, V., Deyholos, M. et al. Plant Mol Biol (2002) 48: 551. doi:10.1023/A:1014875215580

Abstract

Responses to drought and salinity in barley (Hordeum vulgare L. cv. Tokak) were monitored by microarray hybridization of 1463 DNA elements derived from cDNA libraries of 6 and 10 h drought-stressed plants. Functional identities indicated that many cDNAs in these libraries were associated with drought stress. About 38% of the transcripts were novel and functionally unknown. Hybridization experiments were analyzed for drought- and salinity-regulated sequences, with significant changes defined as a deviation from the control exceeding 2.5-fold. Responses of transcripts showed stress-dependent expression patterns and time courses. Nearly 15% of all transcripts were either up- or down-regulated under drought stress, while NaCl led to a change in 5% of the transcripts (24 h, 150 mM NaCl). Transcripts that showed significant up-regulation under drought stress are exemplified by jasmonate-responsive, metallothionein-like, late-embryogenesis-abundant (LEA) and ABA-responsive proteins. Most drastic down-regulation in a category was observed for photosynthesis-related functions. Up-regulation under both drought and salt stress was restricted to ESTs for metallothionein-like and LEA proteins, while increases in ubiquitin-related transcripts characterized salt stress. A number of functionally unknown transcripts from cDNA libraries of drought-stressed plants showed up-regulation by drought but down-regulation by salt stress, documenting how precisely transcript profiles report different growth conditions and environments.

drought stressHordeum vulgaremicroarray hybridizationsalinity stressstress-regulated transcripts

Copyright information

© Kluwer Academic Publishers 2002