Use of Air-Liquid Two-Phase Flow in Hydrophobic Microfluidic Channels for Disposable Flow Cytometers
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This paper describes a disposable flow cytometer that uses an air-liquid two-phase microfluidic system to produce a focused high-speed liquid sample stream of particles and cells. The susceptibility of thin liquid columns to instabilities may suggest that focusing of sample liquids with streams of air would be difficult. The design of channel geometry, control of flow rates, and use of appropriate surface chemistries on the channel walls, however, enabled the generation of thin (15–100 μm) and partially bounded sample streams that were stable and suitable for rapid cell analysis. Using an inverted epi-fluorescence microscope with a photo-multiplier tube, we demonstrated that the system is capable of counting the number of beads and C2C12 myoblast cells. The effects of different flow rates and surface chemistries of the channel walls on the air-liquid two-phase flows were characterized using optical and confocal microscopy. Use of air instead of liquids as a sheath fluid eliminates the need for large sheath liquid reservoirs, and reduces the volume and weight requirements. The low manufacturing cost and high volumetric efficiency make the air-sheath flow cytometer attractive for use as a stand-alone device or as an integrated component of bio-artificial hybrid microsystems.
- K.K. Cavender-Bares, S.L. Frankel, and S.W. Chisholm, Limnol. Oceanography 43(6), 1383-1388 (1998).
- K.A. Criswell, M.R. Bleavins, D. Zielinski, J.C. Zandee, and K.M. Walsh, Cytometry 32(1), 18-27 (1998).
- P.J. Crosland-Taylor, Nature 171, 37-38 (1953).
- A. Cunningham, J. Plankton Res. 12(1), 149-160 (1990).
- G.B.J. Dubelaar and P.L. Gerritzen, Scientia Marina 64(2), 255-265 (2000).
- G.B.J. Dubelaar, P.L. Gerritzen, A.E.R. Beeker, R.R. Jonker, and K. Tangen, Cytometry 37(4), 247-254 (1999).
- D.C. Duffy, J.C. McDonald, O.J.A. Schueller, and G.M. Whitesides, Anal. Chem. 70(23), 4974-4984 (1998).
- D. Fenili and B. Pirovano, Clin. Chem. Lab. Med. 36(12), 909-917 (1998).
- A.Y. Fu, C. Spence, A. Scherer, F.H. Arnold, and S.R. Quake, Nature Biotech. 17(11), 1109-1111 (1999).
- C.L. Harding, D.R. Lloyd, C.M. McFarlane, and M. Al-Rubeai, Biotechnol. Prog. 16(5), 800-802 (2000).
- C.I. Hung, B.J. Ke, G.R. Huang, B.H. Hwei, H.F. Lai, and G.B. Lee, J. Fluids Eng.-Trans. Asme 123(3), 672-679 (2001).
- D.E. Kataoka and S.M. Troian, Nature 402(6763), 794-797 (1999).
- R. Miyake, H. Ohki, I. Yamazaki, and T. Takagi, Jsme Int. J. B-Fluids Thermal Eng. 40(1), 106-113 (1997).
- R. Miyake, H. Ohki, I. Yamazaki, and T. Takagi, Jsme Int. J. B-Fluids Thermal Eng. 43(2), 219-224 (2000).
- S. Niehren, W. Kinzelbach, S. Seeger, and J. Wolfrum, Anal. Chem. 67(15), 2666-2671 (1995).
- M.G. Pollack, R.B. Fair, and A.D. Shenderov, Appl. Phys. Lett. 77(11), 1725-1726 (2000).
- H.M. Shaprio, Practical flow cytometry (Wiley-Liss, New York, 1995).
- D. Sobek, A.M. Young, M.L. Gray, and S.D. Senturia, Proc. IEEE 2, 219-224 (1993).
- H.B. Steen and T. Lindmo, Science 204, 403-404 (1979).
- D.S. Stein, J.A. Korvick, and S.H. Vermund, J. Infect. Dis. 165(2), 352-363 (1992).
- B. Zhao, J.S. Moore, and D.J. Beebe, Science 291(5506), 1023-1026 (2001).
- N.A. Zilmer, M. Godavarti, J.J. Rodriguez, T.A. Yopp, G.M. Lambert, and D.W. Galbraith, Cytometry 20(2), 102-117 (1995).
- Use of Air-Liquid Two-Phase Flow in Hydrophobic Microfluidic Channels for Disposable Flow Cytometers
Volume 4, Issue 2 , pp 141-149
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- air sheath
- flow cytometer
- two-phase flow
- surface chemistry
- cell counting
- Industry Sectors
- Author Affiliations
- 1. Department of Biomedical Engineering, University of Michigan, Ann Arbor, 3304
- 4. GG Brown, 2350 Hayward, Ann Arbor, MI, 48109-2125
- 2. Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2272
- 3. Department of Macromolecular Science and Engineering, University of Michigan, Ann Arbor, Ann Arbor, MI, 48109-2125