Journal of Muscle Research & Cell Motility

, Volume 22, Issue 5, pp 467–475

Tibialis anterior muscles in mdx mice are highly susceptible to contraction-induced injury

Authors

  • Christiana Dellorusso
    • Departments of Human GeneticsUniversity of Michigan
    • Departments of PhysiologyUniversity of Michigan
    • Department of NeurologyUniversity of Washington
  • Robert W. Crawford
    • Departments of Human GeneticsUniversity of Michigan
    • Department of NeurologyUniversity of Washington
  • Jeffrey S. Chamberlain
    • Departments of Human GeneticsUniversity of Michigan
    • Department of NeurologyUniversity of Washington
  • Susan V. Brooks
    • Departments of PhysiologyUniversity of Michigan
    • Institute of GerontologyUniversity of Michigan
Article

DOI: 10.1023/A:1014587918367

Cite this article as:
Dellorusso, C., Crawford, R.W., Chamberlain, J.S. et al. J Muscle Res Cell Motil (2001) 22: 467. doi:10.1023/A:1014587918367

Abstract

Skeletal muscles of patients with Duchenne muscular dystrophy (DMD) and mdx mice lack dystrophin and are more susceptible to contraction-induced injury than control muscles. Our purpose was to develop an assay based on the high susceptibility to injury of limb muscles in mdx mice for use in evaluating therapeutic interventions. The assay involved two stretches of maximally activated tibialis anterior (TA) muscles in situ. Stretches of 40% strain relative to muscle fiber length were initiated from the plateau of isometric contractions. The magnitude of damage was assessed one minute later by the deficit in isometric force. At all ages (2–19 months), force deficits were four- to seven-fold higher for muscles in mdx compared with control mice. For control muscles, force deficits were unrelated to age, whereas force deficits increased dramatically for muscles in mdx mice after 8 months of age. The increase in susceptibility to injury of muscles from older mdx mice did not parallel similar adverse effects on muscle mass or force production. The in situ stretch protocol of TA muscles provides a valuable assay for investigations of the mechanisms of injury in dystrophic muscle and to test therapeutic interventions for reversing DMD.

Copyright information

© Kluwer Academic Publishers 2001