Article

Behavior Genetics

, Volume 32, Issue 1, pp 57-67

Mapping of QTL Influencing Saccharin Consumption in the Selectively Bred Alcohol-Preferring and -Nonpreferring Rat Lines

  • T. ForoudAffiliated withDepartment of Medical and Molecular Genetics, Indiana University School of Medicine Email author 
  • , P. BiceAffiliated withDepartment of Medicine, Indiana University School of Medicine
  • , P. CastelluccioAffiliated withDepartment of Medical and Molecular Genetics, Indiana University School of Medicine
  • , R. BoAffiliated withDepartment of Medicine, Indiana University School of Medicine
  • , A. RitchotteAffiliated withDepartment of Medicine, Indiana University School of Medicine
  • , R. StewartAffiliated withDepartment of Psychology, Purdue School of Science, IUPUI
  • , L. LumengAffiliated withDepartment of Medicine, Indiana University School of MedicineDepartment of Biology, Indiana University School of Medicine
  • , T.-K. LiAffiliated withDepartment of Medicine, Indiana University School of MedicineDepartment of Biology, Indiana University School of Medicine
  • , L. CarrAffiliated withDepartment of Medicine, Indiana University School of MedicineDepartment of Pharmacology, Indianapolis University School of Medicine

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Abstract

The inbred preferring (iP) and nonpreferring (iNP) rat strains were derived from the selectively bred alcohol-preferring (P) and alcohol-nonpreferring (NP) lines. Previously, 381 iP × iNP F2 progeny were generated to identify quantitative trait loci (QTLs) influencing alcohol consumption and preference. Saccharin consumption (ml/48 h) and saccharin intake (ml/kg/day) were also measured in the F2 sample and were significantly correlated with both alcohol consumption and preference (all r ≥ .20, p < .0001), suggesting that there might be some QTLs influencing both saccharin and alcohol phenotypes. We have performed a genome screen using F2 animals with extreme saccharin or alcohol consumption to identify QTLs contributing to saccharin-related phenotypes. Lod scores greater than 2.0 were found on chromosomes 3, 16 and 18 in this sample. Additional genotyping was performed in these regions in the full sample of 381 F2 progeny to further characterize these putative QTLs. On chromosome 3, the maximum lod score in the full sample was 2.7 with saccharin consumption. This QTL appears to overlap with a QTL identified for alcohol consumption in the iP and iNP lines and has pleiotropic effects on both phenotypes. Interestingly, this region of rat chromosome 3 is syntenic with mouse chromosome 2, where a QTL influencing alcohol preference has been previously reported. The QTL on chromosome 16 has a maximum lod score of 4.0 with saccharin intake and 2.6 with saccharin consumption. The QTL on chromosome 18 has a maximum lod score of 2.7 with saccharin consumption. Taken together, these data provide the first results of a genome screen for QTLs contributing to saccharin phenotypes in the rat.

Quantitative trait locus (QTL) saccharin