Molecular and Cellular Biochemistry

, Volume 231, Issue 1, pp 69–74

Identification of TH1 as an interaction partner of A-Raf kinase

  • Xiang L. Yin
  • She. Chen
  • Jian X. Gu

DOI: 10.1023/A:1014437024129

Cite this article as:
Yin, X.L., Chen, S. & Gu, J.X. Mol Cell Biochem (2002) 231: 69. doi:10.1023/A:1014437024129


A-Raf is an important intermediate of the growth factor Ras-MAP kinase pathway. In a two-hybrid screen of human fetal liver cDNA library, TH1 was detected as a new interaction partner of A-Raf. TH1 is a highly conserved and widely expressed protein, which was recently cloned by Bonthron DT group. The binding between A-Raf and TH1 was specific, as no binding between TH1 and B-Raf or c-Raf was observed, and the amino-terminal 162 amino acids in the A-Raf regulatory domain were found to be sufficient for this interaction. This specific interaction may have played a critical role in the activation of A-Raf.

A-Raf kinaseinteractionputative TH1 proteintwo-hybrid system

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Xiang L. Yin
  • She. Chen
  • Jian X. Gu

There are no affiliations available