Plant Molecular Biology

, Volume 49, Issue 1, pp 81–92

Symbiotic and non-symbiotic expression of cgMT1, a metallothionein-like gene from the actinorhizal tree Casuarina glauca

  • Laurent Laplaze
  • Hassen Gherbi
  • Emile Duhoux
  • Katharina Pawlowski
  • Florence Auguy
  • Fathia Guermache
  • Claudine Franche
  • Didier Bogusz
Article

DOI: 10.1023/A:1014415003714

Cite this article as:
Laplaze, L., Gherbi, H., Duhoux, E. et al. Plant Mol Biol (2002) 49: 81. doi:10.1023/A:1014415003714

Abstract

A clone for a type 1 metallothionein (cgMT1) was isolated from a Casuarina glauca nodule cDNA library. The corresponding gene belongs to a small family and is highly expressed in roots and nitrogen-fixing nodules, whereas low expression was observed in aerial parts of the plant. The promoter region of cgMT1 was isolated and fused to the β-glucuronidase (gus) gene. Transgenic Casuarinaceae plants showed that the cgMT1 promoter was most active in roots and in the oldest region of the shoot. In situ hybridization indicated that in nodules cgMT1 transcript is present in mature Frankia-infected cells and in the pericycle. Possible roles for cgMT1 in symbiotic and non-symbiotic tissues are discussed.

actinorhiza,Casuarina glauca, β-glucuronidase genemetallothioneinsymbiosistransgenic plant

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Laurent Laplaze
    • 1
    • 3
  • Hassen Gherbi
    • 2
    • 3
  • Emile Duhoux
    • 3
  • Katharina Pawlowski
    • 4
  • Florence Auguy
    • 3
  • Fathia Guermache
    • 3
  • Claudine Franche
    • 3
  • Didier Bogusz
    • 3
  1. 1.Department of Plant SciencesUniversity of CambridgeCambridgeUK
  2. 2.UMR Amélioration et Santé des Plantes (ASP)Université B. Pascal/INRA 24Aubière CedexFrance
  3. 3.IRD, UR116Montpellier Cedex 1France
  4. 4.Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Biochemie der PflanzeUniversität GöttingenGöttingenGermany