Hydrobiologia

, Volume 464, Issue 1, pp 37–44

Acute inhibition of protease and suppression of growth in zooplankter, Moina macrocopa, by Microcystis blooms collected in Central India

  • Manish K. Agrawal
  • Divya Bagchi
  • Suvendra N. Bagchi
Article

DOI: 10.1023/A:1013946514556

Cite this article as:
Agrawal, M.K., Bagchi, D. & Bagchi, S.N. Hydrobiologia (2001) 464: 37. doi:10.1023/A:1013946514556

Abstract

Cyanobacterial blooms consisting of Microcystis spp., collected from 14 water-bodies in Central India, and an adapted culture, were studied for likely impact on zooplankton community. When fed with single cells of Microcystis from several locations, in mixtures with Chlorella, population growth of the cladoceran Moina macrocopa was suppressed. Microcystis alone was unsuitable as food. In three cases, bloom extracts enhanced mortality of starved zooplankton. Extracts from several sources inhibited protease activity when trypsin or a crude extract from zooplankton served as enzyme source. Upon fractionation by solid-phase extraction, the C-18 passed extract contained the anti-protease and toxic substances for zooplankton, whereas a methanol eluted fraction retained the trypsin inhibitory substance. The study suggests that production of protease inhibitors by cyanobacteria is a factor responsible for feeding inhibition and mortality in zooplankton, which in turn could regulate the community structure of grazers.

Cyanobacterial bloomMicrocystisprotease inhibitiontoxicityzooplankton population growth

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Manish K. Agrawal
    • 1
  • Divya Bagchi
    • 1
  • Suvendra N. Bagchi
    • 2
  1. 1.Department of Biological SciencesRani Durgavati University, JabalpurMadhya PradeshIndia
  2. 2.Department of Biological SciencesRani Durgavati University, JabalpurMadhya PradeshIndia Tel