World Journal of Microbiology and Biotechnology

, Volume 17, Issue 8, pp 761–765

A highly thermostable, alkaline CMCase produced by a newly isolated Bacillus sp. VG1

  • J. Singh
  • N. Batra
  • R.C. Sobti
Article

DOI: 10.1023/A:1013564717107

Cite this article as:
Singh, J., Batra, N. & Sobti, R. World Journal of Microbiology and Biotechnology (2001) 17: 761. doi:10.1023/A:1013564717107

Abstract

An extracellular, highly thermostable and alkaline CMCase was purified from Bacillus sp. VG1 using ion exchange and gel filtration chromatography. Enzyme was optimally produced in a medium containing 1.0% CMC and 0.5% tryptone. The purified CMCase had a pH optimum of 9–10 and a half life of 12 min even at 100 °C. The enzyme activity was reduced by Hg2+ and stimulated by Co2+, Na+ and K+. Various detergents and proteinases moderately inhibited the CMCase activity. The molecular weight studies showed a single band on SDS–PAGE.

AlkalineBacilluscarboxymethyl cellulasedetergentsthermostable

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • J. Singh
    • 1
  • N. Batra
  • R.C. Sobti
    • 1
  1. 1.Department of BiotechnologyPunjab UniversityChandigarh –India