Biogerontology

, Volume 2, Issue 4, pp 261–270

DNA double-strand breaks in mouse kidney cells with age

  • Narendra P. Singh
  • Charles E. Ogburn
  • Norman S. Wolf
  • Gerald van Belle
  • George M. Martin
Article

DOI: 10.1023/A:1013262327193

Cite this article as:
Singh, N.P., Ogburn, C.E., Wolf, N.S. et al. Biogerontology (2001) 2: 261. doi:10.1023/A:1013262327193

Abstract

A Biojector device fitted with a CO2 cartridge was used to prepare single cellsuspensions from kidneys of 12-month-(middle-aged) and 24-month-old (old) C57Bl/6mice. Microgel electrophoresis of DNA fromthese cells revealed a modest but significant7.3% increase (P = 0.04) in DNA double-strand breaks in old mice. This increase is equivalent to the DNA damage induced by 0.1 Gray of X-rays (5 double-strand breaks) in kidney cells of 10-month-old mice, as determined by a standard calibration curve. Greater DNA damage with aging was also positively correlated with higher levels of pathology in the kidneys.

agingBiojectorDNA double-strand breakskidney cellsneutral microgel electrophoresispathological lesions

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Narendra P. Singh
    • 1
  • Charles E. Ogburn
    • 2
  • Norman S. Wolf
    • 2
  • Gerald van Belle
    • 3
  • George M. Martin
    • 4
  1. 1.Department of BioengineeringUniversity of WashingtonSeattleUSA
  2. 2.Department of PathologyUniversity of WashingtonSeattleUSA
  3. 3.Departments of Bio-statistics and Environmental HealthUniversity of WashingtonSeattleUSA
  4. 4.Department of Genetics and Department of PathologyUniversity of WashingtonSeattleUSA