Methods in Cell Science

, Volume 23, Issue 1, pp 141–150

Molecular cytogenetics of introgressive hybridization in plants

  • Kesara Anamthawat-Jónsson

DOI: 10.1023/A:1013182724179

Cite this article as:
Anamthawat-Jónsson, K. Methods Cell Sci (2001) 23: 141. doi:10.1023/A:1013182724179


Introgressive hybridization (introgression) is genetic modification of one species by another through hybridization and repeated backcrossing. Introgression is important in the evolution of flowering plants. It is also important in plant breeding where a desirable trait can be transferred from wild to crop species. One of the most recent advances in molecular techniques for studying hybridization and introgression is in situ hybridization of genomic probes to cytological preparations (GISH, genomic in situ hybridization). The present paper describes a successful GISH protocol for detection of intergenomic introgression in breeding materials and in allopolyploid species. In addition, the paper introduces a new possibility of using dispersed repeats to detect introgression and to gain insights into its molecular basis. The approach is referred to as dFISH for dispersed fluorescence in situ hybridization, and the best candidate for this type of probes is probably a retroelement. Southern hybridization data are also presented to support the effectiveness of GISH and dFISH for introgression mapping.

Dispersed fluorescence in situ hybridization (dFISH)Genomic in situ hybridization (GISH)Introgressive hybridization (introgression)Plant breedingPlant evolution

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Kesara Anamthawat-Jónsson
    • 1
  1. 1.Department of BiologyUniversity of IcelandReykjavíkIceland