, Volume 152, Issue 3, pp 155–158

The effect of fungal ribosome inactivating proteins upon feeding choice in C. freemani, and indications of a mutualistic relationship with A. restrictus. Environmental Mycology

  • Tristan Brandhorst
  • Patrick F. Dowd
  • William R. Kenealy

DOI: 10.1023/A:1013131930192

Cite this article as:
Brandhorst, T., Dowd, P.F. & Kenealy, W.R. Mycopathologia (2001) 152: 155. doi:10.1023/A:1013131930192


Carpophilus freemani beetles' feeding on the fungusAspergillus nidulans was substantially inhibited when A. nidulans was transformed and induced to secrete the ribosome inactivating protein, restrictocin (genetic source: Aspergillus restrictus). No inhibition of feeding was observed when A. nidulans was transformed and induced to produce an inactive form of restrictocin with a single amino-acid substitution in the active site. Similarly, there was no inhibition of feeding upon transgenic strains when the production of restrictocin was not induced. Feeding inhibition of C. freemani by restrictocin requires that the ribonuclease be active and is not due to other characteristics of the protein or the transgenic host fungus.

Aspergillus Carpophilus restrictocin ribonuclease feeding inhibition 

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Tristan Brandhorst
    • 1
  • Patrick F. Dowd
    • 2
  • William R. Kenealy
    • 3
  1. 1.Departments of Pediatrics, University of Wisconsin Medical SchoolUniversity of Wisconsin Hospital and ClinicsMadisonUSA
  2. 2.USDA-Agricultural Research ServicesNCAURPeoriaUSA
  3. 3.J. Whittier BiologicsMadisonUSA