Age and sex influence on oxidative damage and functional status in human skeletal muscle
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A reduction in muscle mass, with consequent decrease in strength and resistance, is commonly observed with advancing age. In this study we measured markers of oxidative damage to DNA, lipids and proteins, some antioxidant enzyme activities as well Ca2+ transport in sarcoplasmic reticulum membranes in muscle biopsies from vastus lateralis of young and elderly healthy subjects of both sexes in order to evaluate the presence of age- and sex- related differences. We found a significant increase in oxidation of DNA and lipids in the elderly group, more evident in males, and a reduction in catalase and glutathione transferase activities. The experiments on Ca2+ transport showed an abnormal functional response of aged muscle after exposure to caffeine, which increases the opening of Ca2+ channels, as well a reduced activity of the Ca2+ pump in elderly males. From these results we conclude that oxidative stress play an important role in muscle aging and that oxidative damage is much more evident in elderly males, suggesting a gender difference maybe related to hormonal factors.
- Akerboom TP and Sies H (1981) Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods Enzymol 77: 373–382.
- Baumgartner RN, Waters DL, Gallagher D, Morley JE and Garry PJ (1999) Predictors of skeletal muscle mass in elderly men and women. Mech Ageing Dev 107: 123–136. CrossRef
- Belia S, Pietrangelo T, Fulle S, Menchetti G, Cecchetti E, Felaco M, Vecchiet J and Fanò G (1998) Sodium nitroprusside, a NO donor modifies Ca2+ transport and mechanical properties in frog skeletal muscle. J Muscle Res Cell Mot 19: 865–876. CrossRef
- Damiani E and Margreth A (1994) Characterization study of the ryanodine receptor and of calsequestrin isoforms of mammalian skeletal muscles in relation to fibre types. J Muscle Res Cell Mot 15: 86–101. CrossRef
- Darnold JR, Vorbeck ML and Martin AP (1990) Effect of aging on the oxidative phosphorylation pathway. Mech Ageing Dev 53: 157–167. CrossRef
- Fanò G, Belia S, Fulle S, Angelella P, Panara F, Marsili V and Pascolini R (1989) Functional aspects of calcium transport in sarcoplasmic reticulum vesicles derived from frog skeletal muscle treated with saponin. J Muscle Res Cell Mot 10: 326–330. CrossRef
- Green PS and Simpkins JW (2000) Neuroprotective effects of estrogens: potential mechanisms of action. Int J Devl Neurosci 18: 347–358. CrossRef
- Greenwald RA (1985) Therapeutic benefits of oxygen radical scavenger treatments remain unproven. Free Rad Biol Med 1: 173–177. CrossRef
- Habig WH and Jakoby WB (1981) Assays for differentiation of glutathione S-transferases. Methods Enzymol 77: 398–405.
- Hall ED, Pazara KL and Linseman KL (1991) Sex differences in postischemic neuronal necrosis in gerbils. J Cerebral Blood Flow Metab 11: 292–298.
- Halliwell B and Chirico S (1993) Lipid peroxidation: its mechanism, measurement, and significance. Am J Clin Nutr 57 (suppl): 715S–725S.
- Hussey AJ, Kerr LA, Cronshaw AD, Harrison DJ and Hayes JD (1991) Variation in the expression of Mu-class glutathione S-transferase isoenzymes from human skeletal muscle. Evidence for the existence of heterodimers. Biochem J 273: 323–332.
- L'Abbé MR and Fisher WF (1986) An automated method for the determination of Cu, Zn superoxide dismutase in plasma and erythrocytes using an ABA discrete analyzer. Clin Biochem 19: 175–178. CrossRef
- Lamberts SWJ, van den Beld AW and van der Lely AJ (1997) The endocrinology of aging. Science 278: 419–424. CrossRef
- Lawrence RA and Burk RF (1976) Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71: 952–958. CrossRef
- Lowry OH, Rosebrough NJ, Farr ASL and Randall RJ (1951) Protein measurement with Folin Phenol Reagent. J Biol Chem 193: 265–275.
- Margreth A, Damiani E and Bortoloso E (1999) Sarcoplasmic reticulum in aged skeletal muscle. Acta Physiol Scand 167: 331–338. CrossRef
- Marsili V, Mancinelli L, Menchetti G, Fulle S, Baldoni F and Fanò G (1992) S-100ab increases Ca2+ release in purified sarcoplasmic vesicles of frog skeletal muscle. J Muscle Res Cell Mot 13: 511–515. CrossRef
- Mecocci P, Fanò G, Fulle S, MacGarvey U, Shinobu L, Polidori MC, Cherubini A, Vecchiet J, Senin U and Beal MF (1999) Age-dependent increases of oxidative damage to DNA, lipids, and proteins in human skeletal muscle. Free Radic Biol Med 26: 303–308. CrossRef
- Narayanan N, Jones DL, Xu A and Yu JC (1996) Effects of aging on sarcoplasmic reticulum function and contraction duration in skeletal muscles of the rat. Am J Physiol 271: C1032–C1040.
- Neder JA, Nery LE, Silva AC, Andreoni S and Whipp BJ (1999) Maximal aerobic power and leg muscle mass and strength related to age in non-athletic males and females. Eur J Appl Physiol 79: 522–530. CrossRef
- Pansarasa O, Bertorelli L, Vecchiet J, Felzani G and Marzatico F (1999) Age-dependent changes of antioxidant activities and markers of free radical damage in human skeletal muscle. Free Radic Biol Med 27: 617–622. CrossRef
- Pansarasa O, Castagna L, Colombi B, Vecchiet J, Felzani G and Marzatico F (2000) Age and sex differences in human skeletal muscle: role of reactive oxygen species. Free Radic Res 33(3): 287–293.
- Ramos-Martinez JI, Bartolomè TR and Pernas RV (1983) Purification and properties of glutathione reductase from hepatopancreas of mytilus edulis. M Comp Biochem Physiol 75B: 689–692. CrossRef
- Renganathan M, Messi ML and Delbono O (1997) Dihydropyridine receptor-ryanodine receptor uncoupling in aged skeletal muscle. J Membr Biol 157: 247–253. CrossRef
- Reznik AZ and Packer L (1994) Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 233: 357–363.
- Rock E, Mammar MS, Vignon X, Thomas MA and Viret J (1990) Abnormal fluidity state in membrane of malignant hyperthermia pig skeletal muscle. Arch Biochem Biophys 281: 36–40. CrossRef
- Short KR and Nair KS (1999) Mechanisms of sarcopenia of aging. J Endocrinol Invest 22: 95–105.
- Sohal RS and Sohal BH (1991) Hydrogen peroxide release by mitochondria increases during aging. Mech Ageing Dev 57: 187–202. CrossRef
- Sohal RS, Agarwal S, Candas M, Forster MJ and Lal H (1994) Effect of age and caloric restriction on DNA oxidative damage in different tissues of C57BL/6 mice. Mech Ageing Dev 76: 215–224. CrossRef
- Stuart J, Pessah IN, Faver TG and Ascbramson JJ (1992) Photooxidation of skeletal muscle sarcoplasmic reticulum induces rapid calcium release. Archiv Biochem Biophys 292: 512–521. CrossRef
- Treves S, Scutari E, Robert M, Groh S, Ottolia M, Prestipino G, Ronjat M and Zorzato F (1997) Interaction of S100A1 with the Ca2+ release channel (ryanodine receptor) of skeletal muscle. Biochem 36: 11496–11503. CrossRef
- Wolfson L, Judge J, Whipple R and King M (1995) Strength is a major factor in balance, gait and the occurrence of falls. J Gerontol Medical Sci 50A: 64–67.
- Age and sex influence on oxidative damage and functional status in human skeletal muscle
Journal of Muscle Research & Cell Motility
Volume 22, Issue 4 , pp 345-351
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- Author Affiliations
- 1. Laboratorio Interuniversitario di Miologia, sez. Chieti, Università “G. D'Annunzio”, Via dei Vestini, 66013, Chieti, Italy
- 2. Dip. Medicina Clinica e Sperimentale sez, Gerontologia e Geriatria, Italy
- 3. Laboratorio Interuniversitario di Miologia, sez. Perugia, Università di Perugia, Italy
- 4. Department of Neurology and Neuroscience, New York Presbyterian Hospital, Cornell University, New York, USA